TY - JOUR
AU - Weiwei Dai, Stéphane Panserat, Frédéric Terrier, Iban Seiliez, Sandrine Skiba-Cassy
AB - Abstract Our aim was to investigate the potential role of TOR (target of rapamycin) signaling pathway in the regulation of hepatic glucose metabolism in rainbow trout. Fasted fish were first treated with a single intraperitoneal injection of rapamycin or vehicle and then submitted to a second intraperitoneal administration of glucose 4 h later. Our results revealed that intraperitoneal administration of glucose induced hyperglycemia for both vehicle and rapamycin treatments, which peaked at 2 h. Plasma glucose level in vehicle-treated fish was significantly higher than in rapamycin-treated fish at 8 and 17 h, whereas it remained at the basal level in rapamycin-treated fish. Glucose administration significantly enhanced the phosphorylation of Akt and ribosomal protein S6 kinase (S6K1) in vehicle-treated fish, while rapamycin completely abolished the activation of S6K1 in rapamycin-treated fish, without inhibiting the phosphorylation of Akt on Thr-308 or Ser-473. Despite the lack of significant variation in phosphoenolpyruvate carboxykinase mRNA abundance, mRNA abundance for glucokinase (GK), glucose 6-phosphatase (G6Pase) I and II, and fructose 1,6-bisphosphatase (FBPase) was reduced by rapamycin 17 h after glucose administration. The inhibition effect of rapamycin on GK and FBPase was further substantiated at the activity level. The suppression of GK gene expression and activity by rapamycin provided the first in vivo evidence in fish that glucose regulates hepatic GK gene expression and activity through a TORC1-dependent manner. Unlike in mammals, we observed that acute rapamycin treatment improved glucose tolerance through the inhibition of hepatic gluconeogenesis in rainbow trout. target of rapamycin rapamycin gluconeogenesis glucokinase glucose homeostasis rainbow trout Copyright © 2014 the American Physiological Society View Full Text Previous Next Back to top Table of Contents Back Matter (PDF) Ed Board (PDF) Keywords target of rapamycin Rapamycin gluconeogenesis glucokinase glucose homeostasis rainbow trout Article Abstract MATERIALS AND METHODS RESULTS DISCUSSION GRANTS DISCLOSURES AUTHOR CONTRIBUTIONS ACKNOWLEDGMENTS REFERENCES Figures & Data Info PDF Alert me when this article is cited Alert me if a correction is posted Email Thank you for your interest in spreading the word on Regulatory, Integrative and Comparative Physiology. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. 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Your Personal Message Print Citation Tools Acute rapamycin treatment improved glucose tolerance through inhibition of hepatic gluconeogenesis in rainbow trout ( Oncorhynchus mykiss ) Weiwei Dai , Stéphane Panserat , Frédéric Terrier , Iban Seiliez , Sandrine Skiba-Cassy American Journal of Physiology - Regulatory, Integrative and Comparative Physiology Nov 2014, 307 (10) R1231-R1238; DOI: 10.1152/ajpregu.00166.2014 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Request Permissions Share Acute rapamycin treatment improved glucose tolerance through inhibition of hepatic gluconeogenesis in rainbow trout ( Oncorhynchus mykiss ) Weiwei Dai , Stéphane Panserat , Frédéric Terrier , Iban Seiliez , Sandrine Skiba-Cassy American Journal of Physiology - Regulatory, Integrative and Comparative Physiology Nov 2014, 307 (10) R1231-R1238; DOI: 10.1152/ajpregu.00166.2014 Permalink: Copy View Full Page PDF Tweet Widget Facebook Like Google Plus One Reddit CiteULike Mendeley StumbleUpon More in this TOC Section Chronic anemic hypoxemia increases plasma glucagon and hepatic PCK1 mRNA in late-gestation fetal sheep Protein kinase A induces UCP1 expression in specific adipose depots to increase energy expenditure and improve metabolic health Insulin/glucose induces natriuretic peptide clearance receptor in human adipocytes: a metabolic link with the cardiac natriuretic pathway Show more Obesity, Diabetes and Energy Homeostasis Related Articles No related articles found. 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TI - Acute rapamycin treatment improved glucose tolerance through inhibition of hepatic gluconeogenesis in rainbow trout (Oncorhynchus mykiss)
JF - AJP - Regulatory, Integrative and Comparative Physiology
DO - 10.1152/ajpregu.00166.2014
DA - 2014-11-15
UR - https://www.deepdyve.com/lp/the-american-physiological-society/acute-rapamycin-treatment-improved-glucose-tolerance-through-1CPWYZGMv4
SP - R1231
VL - 307
IS - 10
DP - DeepDyve
ER -