TY - JOUR
AU - Nelson, Marla, A.
AB - Abstract A simple, accurate, and specific liquid chromatographic procedure is described for the determination of gentamicin in 50 µL of serum. Gentamicin and sisomicin (IS) were converted into their trinitrophenyl derivatives by reaction with a water soluble derivatizing agent at 70°C for 30 minutes. The derivatives were extracted from the crude mixture with chloroform, which was separated, evaporated, and the residue reconstituted in acetonitrlle. Gentamicin was separated Into three major isomers (C1, C2, and C1) on a reversed-phase octyl column by eluting with a mobile phase. The eluted compounds were detected at 340 nm and quantified from their peak heights or peak areas. Chromatography was complete in ◂11.0 minutes. The lower limit of detection for gentamicin was ◂0.5 mg/L, analytical recoveries varied from 96.6% to 99.3%, linearity extended to 15.0 rag/L, and day-to-day precision was between 2.2% to 2.9%. This content is only available as a PDF.
TI - Liquid Chromatographic Determination of Gentamicin in Serum with Spectrophotometric Detection
JO - Journal of Analytical Toxicology
DO - 10.1093/jat/7.6.283
DA - 1983-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/liquid-chromatographic-determination-of-gentamicin-in-serum-with-0inRc0xA20
SP - 283
EP - 285
VL - 7
IS - 6
DP - DeepDyve
ER -