TY - JOUR
AU - Abeliovich,, Aharon
AB - Abstract A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. europaea and a Nitrosomonas spp. isolated from a local oxidation pond. The primers failed to amplify DNA from the genomes of the ammonia oxidiser Nitrosolobous multiformis, the nitrite oxidiser Nitrococcus mobilis as well as from the genomes of other unrelated heterotrophic bacteria. These DNA sequences could be amplified from 0.01 ng of N. europaea genomic DNA or from 100 intact cells, and it was possible to detect Nitrosomonas DNA in a DNA mixture extracted from water samples drawn from a local oxidation pond. Nitrifying bacteria, Nitrosomonas europaea, Nitrosomonas spp, Polymerase chain reaction, Oxidation pond References 1 Bock E. Koops H.S. Harms H. ( 1989 ) Nitrifying bacteria In Autotrophic Bacteria Schlegel H.G. Bowien B., Eds) pp 81 – 96 Springer Verlag , Berlin . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 2 Focht D.D. Verstraete W. ( 1977 ) Biochemical ecology of nitrification and denitrification . Vol. 1 , In Advances in Microbial Ecology , pp 135 – 214 Plenum Press , New York . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 3 Hooper A.B. ( 1989 ) Biochemistry of the nitrifying lithoautotrophic bacteria In Autotrophic Bacteria Schlegel H.G. Bowien B., Eds) pp 239 – 265 Springer Verlag , Berlin . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 4 Saiki R.K. Scharf S. Faloona F. Mullis K.B. Horn G.T. Erlich H.A. Arnheim N. ( 1985 ) Enzymatic amplification of b-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Science , 230 , 1350 – 1354 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Steffan R.J. Atlas R.M. ( 1991 ) Polymerase chain reaction: Application in environmental microbiology Ann. Rev. Microbiol. , 45 , 137 – 161 . Google Scholar Crossref Search ADS WorldCat 6 Abeliovich A. ( 1987 ) Nitrifying bacteria in wastewater reservoirs Appl. Environ. Microbiol. , 53 , 754 – 760 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 7 Ausubel F.M. Brent R. Kingston R.E. Moore D.D. Smith J.A. Sideman J.G. Struhl K. ( 1987 ) Current Protocols in Molecular Biology John Wiley and Sons Inc , New York . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 8 Yamanaka T. Shimra M. Takahashi K. Shibasaka M. ( 1979 ) Highly purified hydroxylamine oxidoreductase derived from Nitrosomonas europaea J. Biochem. , 86 , 1101 – 1108 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Horn B.A.L. Chantler S.M. ( 1980 ) Production of reagent antibodies Meth. Enzymol. , 70 , 104 – 150 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Sanger F. Nicklen S. Coulson A.R. ( 1977 ) DNA sequencing with chain-terminating inhibitors . 74 , In Proc. Natl. Acad. Sci. USA , pp 5463 – 5467 . 11 Henikoff S. ( 1984 ) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing Gene , 28 , 351 – 359 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Sarker G. Sommer S.S. ( 1990 ) Shedding light on PCR contamination Nature , 343 , 27 . Google Scholar Crossref Search ADS PubMed WorldCat 13 Sambrook J. Fritsch E.F. Maniatis T. ( 1989 ) In Molecular Cloning: a Laboratory Manual Cold Spring Harbor Laboratory , Cold Spring Harbor, NY . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 14 Tsai Y-L. Olson B.H. ( 1992 ) Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction Appl. Environ. Microbiol. , 58 , 2292 – 2295 . Google Scholar PubMed OpenURL Placeholder Text WorldCat This content is only available as a PDF. © 1994 Federation of European Microbiological Societies
TI - Detection of Nitrosomonas spp. by polymerase chain reaction
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1994.tb07029.x
DA - 1994-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/detection-of-nitrosomonas-spp-by-polymerase-chain-reaction-0f10mOAf3c
SP - 191
EP - 194
VL - 120
IS - 1-2
DP - DeepDyve
ER -