TY - JOUR
AU1 - Baysa, Anton
AU2 - Fedorov, Anton
AU3 - Kondratov, Kirill
AU4 - Ruusalepp, Arno
AU5 - Minasian, Sarkis
AU6 - Galagudza, Michael
AU7 - Popov, Maxim
AU8 - Kurapeev, Dmitry
AU9 - Yakovlev, Alexey
AU1 - Valen, Guro
AU1 - Kostareva, Anna
AU1 - Vaage, Jarle
AU1 - Stensløkken, Kåre-Olav
AB - During heart surgery with cardiopulmonary bypass (CPB), the release of mitochondrial (mtDNA) and nuclear DNA (nDNA) and their association to extracellular vesicles were investigated. In patients undergoing elective coronary artery bypass grafting (CABG, n = 12), blood was sampled before, during, and after surgery from peripheral artery, pulmonary artery, and the coronary sinus. Plasma was separated in three fractions: microvesicles, exosomes, and supernatant. mtDNA and nDNA were measured by qPCR. mtDNA and nDNA levels increased after start of surgery, but before CPB, and increased further during CPB. mtDNA copy number was about 1000-fold higher than nDNA. mtDNA was predominantly localized to the vesicular fractions in plasma, whereas nDNA was predominantly in the supernatant. The amount of free mtDNA increased after surgery. There was no net release or disappearance of DNAs across the pulmonary, systemic, or coronary circulation. Extracellular DNAs, in particular mtDNA, may be important contributors to the whole-body inflammation during CPB.
TI - Release of Mitochondrial and Nuclear DNA During On-Pump Heart Surgery: Kinetics and Relation to Extracellular Vesicles
JF - Journal of Cardiovascular Translational Research
DO - 10.1007/s12265-018-9848-3
DA - 2018-12-12
UR - https://www.deepdyve.com/lp/springer-journals/release-of-mitochondrial-and-nuclear-dna-during-on-pump-heart-surgery-0bFViBS9U3
SP - 184
EP - 192
VL - 12
IS - 3
DP - DeepDyve
ER -