TY - JOUR
AU - Park, Enoch Y.
AB - Early diagnosis of dengue infection by detecting the dengue virus non-structural protein 1 (DENV-NS1) is important to the patients to initiate speedy treatment. Enzyme-linked immunosorbent assay (ELISA)-based NS1 detection and RT-PCR are time-consuming and too complex to be employed in remote areas of dengue-endemic countries. Meanwhile, those of NS1 rapid test by lateral flow assay suffer from low detection limit. Electrochemical-based biosensors using screen-printed gold electrodes (SPGEs) have become a reliable detection method to convey both ELISA’s high sensitivity and rapid test portability. In this research, we developed an electrochemical biosensor for DENV-NS1 detection by employing polydopamine (PDA)-modified SPGE. The electrodeposition of PDA on the surface of SPGE serves as a bioconjugation avenue for anti-NS1 antibody through a simple and low-cost immobilization procedure. The biosensor performance was evaluated to detect DENV-NS1 protein in PBS and human serum through a differential pulse voltammetric (DPV) technique. The developed sensing platform displayed a low limit of detection (LOD) of 1.63 pg mL–1 and a wide linear range of 10 pg mL–1 to 1 ng mL–1 (R2 ∼ 0.969). The sensing platform also detected DEV-NS1 from four different serotypes in the clinical samples collected from dengue patients in India and Indonesia, with acceptable sensitivity, specificity, and accuracy values of 90.00%, 80.95%, and 87.65%, respectively. This result showcased the facile and versatile method of PDA coating onto the surface of screen-printed gold electrodes for a miniaturized point-of-care (PoC) detection device.Graphical Abstract[graphic not available: see fulltext]
TI - Point-of-care dengue detection: polydopamine-modified electrode for rapid NS1 protein testing for clinical samples
JF - Microchimica Acta
DO - 10.1007/s00604-024-06259-7
DA - 2024-04-01
UR - https://www.deepdyve.com/lp/springer-journals/point-of-care-dengue-detection-polydopamine-modified-electrode-for-0Z0fplLjXF
VL - 191
IS - 4
DP - DeepDyve
ER -