TY - JOUR
AU - Doniger, Jay
AB - Primary human oral keratinocytes were transformed by transfection with recombinant human papillomavirus type 16 (HPV-16)DNA, and two transformed cell lines named human oral keratinocytes-16A and -16B (HOK-16A and HOK-16B) were established. While normal cells and cells transfected with vector only exhibited a limited Wespan, the HOK-16A and HOK-16B lines demonstrated immortality and altered morphology from their normal counterpart. The HOK-16A and HOK-16B lines contained ˜40 and ˜25 copies of intact HPV-16 DNA as integrated form per cell respectively, and both cell lines exprpssed several viral specific poly(A+) RNAs. Notably these cell lines also overexpressed cellular myc proto-oncogene in comparison with the normal counterpart. However, the immortalized cell lines were not able to produce tumors in nude mice, indicating that the cells are partially transformed. The HOK-16A and HOK-16B lines are, therefore, useful for investigating the multistep molecular events of oral carcinogenesis.
TI - Immortalization of normal human oral keratinocytes with type 16 human papillomavirus
JF - Carcinogenesis
DO - 10.1093/carcin/12.9.1627
DA - 1991-09-01
UR - https://www.deepdyve.com/lp/oxford-university-press/immortalization-of-normal-human-oral-keratinocytes-with-type-16-human-0NVHgf8Df4
SP - 1627
EP - 1631
VL - 12
IS - 9
DP - DeepDyve
ER -