TY - JOUR
AU - Lee, Robert T, Y
AB - Abstract We describe a new method for measuring total bilirubin in serum. Nonionic, cationic, or anionic surfactants can be used as solubilizing agents to promote the diazo coupling of indirect-reacting bilirubin. A representative surfactant—azobilirubin system is illustrated, in which absorbance is maximum at 560 nm and linear to a concentration of 200 mg of bilirubin per liter. At pH 2.5 and in the presence of Du ponol (an anionic surfactant), bilirubin is completely coupled within 6 mm at 37 °C. All ingredients required for an assay are combined in two dry reagents, which are stable for a year at room temperature. Sensitivity is comparable to that of procedures routinely used in clinical laboratories; precision, linearity, and stability of the reagents in solution are excellent. Results for fresh sera correlated well with those obtained by the Jendrassik—Grof method. Hemolysis is minimized and turbidity eliminated. Jendrassik—Grof method compared, error evaluation as compared with four methods This content is only available as a PDF. © 1974 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Detection and Measurement of Total Bilirubin in Serum, with Use of Surfactants as Solubilizing Agents
JF - Clinical Chemistry
DO - 10.1093/clinchem/20.4.447
DA - 1974-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/detection-and-measurement-of-total-bilirubin-in-serum-with-use-of-0NDb3cmYDV
SP - 447
EP - 453
VL - 20
IS - 4
DP - DeepDyve
ER -