TY - JOUR AB - Review Structures of multisubunit membrane complexes with the CRYO ARM Christoph Gerle, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, Akihiro Kawamoto, Ken Yokoyama, Keiichi Namba, Genji Kurisu and Takayuki Kato MiniAbstract:Structuredeterminationofthreelarge,multisub- unit membrane complexes using the CRYO ARM 200 from JEOL exemplifytheabilityofmodern200keVtransmissioncryoelec- tron microscopes to provide significant insight into membrane biology. Future directions and demands for affordable single particle cryo-EM of membrane proteins are discussed. Microscopy 2022 71:5 249–261; doi:10.1093/jmicro/dfac037 Articles Whole-cellobservationofZIO-stainedGolgiapparatusinrathepatocytes with serial block-face scanning electron microscope, SBF-SEM KoheiJohkura, NobuteruUsuda, YoshihiroTanaka, Motoaki Fukasawa, Kazuyoshi Murata, Toru Noda and Nobuhiko Ohno Mini Abstract: We firstly reported 3D-reconstruction of a biological specimen by SBF-SEM after histochemical analysis of an organelle. This combination of the methods enabled semi-automatic processing of many images to obtain whole cell 3D-reconstruction, and quantitation of organelles. 3D- reconstruction of whole hepatocytes visualized characteristic localization of the Golgi apparatus along bile canaliculi. Microscopy 2022 71:5 262–270; doi:10.1093/jmicro/dfac024 Malaria parasite classification framework using a novel channel squeezed and boosted CNN Saddam Hussain Khan, Najmus Saher Shah, Rabia Nuzhat, Abdul Majid, Hani Alquhayz and Asifullah Khan Mini Abstract: A novel STM-SB-RENet CNN is proposed with the concept of new STM blocks and channel SB TI - In This Issue JF - Microscopy DO - 10.1093/jmicro/dfac043 DA - 2022-10-06 UR - https://www.deepdyve.com/lp/oxford-university-press/in-this-issue-04jYPp3irR SP - 1 EP - 3 VL - 71 IS - 5 DP - DeepDyve ER -