TY - JOUR
AU - Rock., G.
AB - The cytotoxic reactivity of 18 predefined class I HLA serum antibodies was compared with that ofantibody preparations containing anticoagulants. ACD-A, EDTA, 4% citrate and heparin plasmas all showed lowercytotoxicity than serum antibodies. Recalcification of both platelet-rich and platelet-poor ACD-A plasma did notfully restore the antibody reactivity, suggesting a detrimental effect of calcium chelation. This effect was exclusive ofvolume or of any platelet or plasma protein involvement. The changes in pH contributed to the lower reactivity andto the increased lympholytic effect, whereas adjusting the pH toward the serum value improved the reactivity.Heat-inactivated antibodies showed only a slightly reduced cytotoxicity. Heparin had the least effect of all anticoagulantson the reactivity, although in heparin there was a definite dose-dependent decline in cytotoxic titer which wasprobably related to anticomplementary activity. Calcium chelators, such as EDTA and citrate, showed markedcytotoxic inhibition at half the usual complement concentration. This effect was more pronounced when theanticoagulant and lymphocytes were incubated prior to cytotoxicity testing. At the complement concentrations used,the inhibitory effects of the citrate anticoagulants appeared to be primarily calcium-related. Inhibition tests, serialtitrations and testing of varying calcium concentrations confirmed the superiority of serum as antibody source.
TI - Is Serum the Optimal Source for HLA Antibodies?
JF - Vox Sanguinis
DO - 10.1159/000461618
DA - 2017-01-01
UR - https://www.deepdyve.com/lp/karger/is-serum-the-optimal-source-for-hla-antibodies-02nj4EUege
SP - 89
EP - 94
VL - 52
IS - 1-2
DP - DeepDyve
ER -