TY - JOUR AU - Serrano, Geidy G AB -

Determining RNA integrity is a critical quality assessment tool for gene expression studies where the experiment9s success is highly dependent on sample quality. Since its introduction in 1999, the gold standard in the scientific community has been the Agilent 2100 Bioanalyzer9s RNA Integrity Number (RIN) which uses a 1-10 value system with 1 being the most degraded to 10 being the most intact. In 2015, Agilent launched the 4200 Tapestation9s RIN equivalent and reported a strong correlation of r2 of 0.936 and median error < ± 0.4 RIN units. To evaluate this claim, we compared the Agilent9s 4200 Tapestation9s RIN equivalent (RINe) and DV200 to the Agilent 2100 Bioanalyzer9s RIN for 183 parallel RNA samples. In our study, using RNA from a total of 183 human postmortem brain samples, we found that the RIN and RINe values only weakly correlated with an r2 of 0.393 and an average difference of 3.2 RIN units. DV200 also only weakly correlated with RIN (r2 of 0.182) and RINe (r2 of 0.347). Finally, when applying a cut-off value of 6.5 for both metrics, we found that 95.6% of samples passed for RIN, while only 23.5% passed with RINe. Our results suggest that even though RIN (Bioanalyzer) and RINe (Tapestation) use the same 1-10 value system, they should not be used interchangeably, and cut-off values should be calculated independently.

TI - Measuring up: A Comparison of Tapestation 4200 and Bioanalyzer 2100 as Measurement Tools for RNA Quality in Postmortem Human Brain Samples JF - medRxiv DO - 10.1101/2023.07.03.23291969 DA - 2023-07-06 UR - https://www.deepdyve.com/lp/medrxiv/measuring-up-a-comparison-of-tapestation-4200-and-bioanalyzer-2100-as-011ksDofXM SP - 23291969 DP - DeepDyve ER -