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Chemical and biochemical approaches to the production of 7-hydroxylated C 19 -steroids

Chemical and biochemical approaches to the production of 7-hydroxylated C 19 -steroids Abstract Interest has developed into 7-hydroxylated derivatives of 3β-hydroxylated C 19 -steroids, such as dehydroepiandrosterone (DHEA) and epiandrosterone because of their effects on inflammation, immune response, and cell repair. These steroids are not currently available from commercial sources, and it is necessary to produce them for relevant studies. We report here the chemical and biochemical approaches that were used for their production. Simplified chemical approaches lead to the production of 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone in gram quantities, which are made available to researchers. Biochemical approaches were used to produce isotope-labeled compounds. Thus, 2 H-, 3 H-, and 14 C-labeled 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone could be produced in quantities sufficient for use in investigations into their mode of action. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Hormone Molecular Biology and Clinical Investigation de Gruyter

Chemical and biochemical approaches to the production of 7-hydroxylated C 19 -steroids

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References (36)

Publisher
de Gruyter
Copyright
Copyright © 2012 by the
ISSN
1868-1883
eISSN
1868-1891
DOI
10.1515/hmbci-2012-0013
pmid
25436687
Publisher site
See Article on Publisher Site

Abstract

Abstract Interest has developed into 7-hydroxylated derivatives of 3β-hydroxylated C 19 -steroids, such as dehydroepiandrosterone (DHEA) and epiandrosterone because of their effects on inflammation, immune response, and cell repair. These steroids are not currently available from commercial sources, and it is necessary to produce them for relevant studies. We report here the chemical and biochemical approaches that were used for their production. Simplified chemical approaches lead to the production of 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone in gram quantities, which are made available to researchers. Biochemical approaches were used to produce isotope-labeled compounds. Thus, 2 H-, 3 H-, and 14 C-labeled 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone could be produced in quantities sufficient for use in investigations into their mode of action.

Journal

Hormone Molecular Biology and Clinical Investigationde Gruyter

Published: Jun 1, 2012

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