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Hydroxyl Radical Activity Associated with the Growth of White-Rot Fungi

Hydroxyl Radical Activity Associated with the Growth of White-Rot Fungi Introduction Recently, Backa et al. (1992) reported that brown-rot fungi produce hydroxyl radicals during growth and that these radicals may contribute significantly to the initial degradation of wood. A selective and sensitive chemiluminescence method (Reitberger and Gierer 1988) was used to detect these strongly oxidative species. The present work employs the same analytical method to demonstrate the formation of hydroxyl radicals during the growth of white-rot fungi, and examines the possible role of hydroxyl radicals in wood degradation by these fungi. Data are interpreted in terms of a general concept describing fungalmediated wood degradation, and tentative explanations are given for the different wood-degrading activities of brown- and white-rot fungi. Experimental Materials The following fungal strains, all of which cause typical white-rot in wood, were used: Phanerochaete chrysosporium (P127-1), Lentinus edodes (IFO 7123) and Coriolus versicolor (A-361). Chemicals, wood shavings, agar and liquid media were similar to those used in the earlier study of brown-rot fungi (Antrodia xantha and Postia placenta) (Backa etal. 1992). Hemin was purchased-from Sigma and catalase (20 U/mg) was obtained from Millipore Corp. Lignin peroxidase was kindly provided by Mr. Bert Petterson, STFI, Stockholm, Sweden. Methods Details of -irradiation and fungal treatment of wood shavings http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Holzforschung - International Journal of the Biology, Chemistry, Physics and Technology of Wood de Gruyter

Hydroxyl Radical Activity Associated with the Growth of White-Rot Fungi

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References (35)

Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
0018-3830
eISSN
1437-434X
DOI
10.1515/hfsg.1993.47.3.181
Publisher site
See Article on Publisher Site

Abstract

Introduction Recently, Backa et al. (1992) reported that brown-rot fungi produce hydroxyl radicals during growth and that these radicals may contribute significantly to the initial degradation of wood. A selective and sensitive chemiluminescence method (Reitberger and Gierer 1988) was used to detect these strongly oxidative species. The present work employs the same analytical method to demonstrate the formation of hydroxyl radicals during the growth of white-rot fungi, and examines the possible role of hydroxyl radicals in wood degradation by these fungi. Data are interpreted in terms of a general concept describing fungalmediated wood degradation, and tentative explanations are given for the different wood-degrading activities of brown- and white-rot fungi. Experimental Materials The following fungal strains, all of which cause typical white-rot in wood, were used: Phanerochaete chrysosporium (P127-1), Lentinus edodes (IFO 7123) and Coriolus versicolor (A-361). Chemicals, wood shavings, agar and liquid media were similar to those used in the earlier study of brown-rot fungi (Antrodia xantha and Postia placenta) (Backa etal. 1992). Hemin was purchased-from Sigma and catalase (20 U/mg) was obtained from Millipore Corp. Lignin peroxidase was kindly provided by Mr. Bert Petterson, STFI, Stockholm, Sweden. Methods Details of -irradiation and fungal treatment of wood shavings

Journal

Holzforschung - International Journal of the Biology, Chemistry, Physics and Technology of Wood – de Gruyter

Published: Jan 1, 1993

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