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Effect of Different Columns and Internal Standards on the Quality Assurance of the Gas Chromatographic Determination of Blood Ethanol

Effect of Different Columns and Internal Standards on the Quality Assurance of the Gas... Introduction The clinical and forensic laboratory is responsible for providing results which are as precise and accurate as possible (1 --2). It is generally accepted that two methods, an oxido-reductimetric and a gas Chromatographie method for the determination of blood ethanol concentration, are statuary in forensic practice (3--4). Gas chromatography is generally recommended for identification and quantitation of volatile polar organic compounds such as ethanol in body fluids (5--6). Different Chromatographie strategies for blood ethanol determination can be adopted for clinical and forensic purposes (7). Sampling techniques include headspace analysis (8 -- 10), liquid-liquid extractions and direct injection of body fluids. If both blood and urine samples are to be analysed, the direct injection of diluted plasma samples, which minimizes sample handling and allows rapid feedback of data, is preferred (11 -- 12). Syringe blockage can be minimized by using removable needles or by washing the syringe. The injection port must be regularly cleaned of residues from biological samples. Eiir. J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 11 Quality assurance has become an essential element of clinical and forensic science. All aspects of performance should be monitored to ensure that the highest possible standards are http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Effect of Different Columns and Internal Standards on the Quality Assurance of the Gas Chromatographic Determination of Blood Ethanol

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References (16)

Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.11.773
Publisher site
See Article on Publisher Site

Abstract

Introduction The clinical and forensic laboratory is responsible for providing results which are as precise and accurate as possible (1 --2). It is generally accepted that two methods, an oxido-reductimetric and a gas Chromatographie method for the determination of blood ethanol concentration, are statuary in forensic practice (3--4). Gas chromatography is generally recommended for identification and quantitation of volatile polar organic compounds such as ethanol in body fluids (5--6). Different Chromatographie strategies for blood ethanol determination can be adopted for clinical and forensic purposes (7). Sampling techniques include headspace analysis (8 -- 10), liquid-liquid extractions and direct injection of body fluids. If both blood and urine samples are to be analysed, the direct injection of diluted plasma samples, which minimizes sample handling and allows rapid feedback of data, is preferred (11 -- 12). Syringe blockage can be minimized by using removable needles or by washing the syringe. The injection port must be regularly cleaned of residues from biological samples. Eiir. J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 11 Quality assurance has become an essential element of clinical and forensic science. All aspects of performance should be monitored to ensure that the highest possible standards are

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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