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The Reliability of a Mechanized Procedure (Perkin-Elmer C4) for the Enzymatic Determination of Uric Acid According to Kageyama

The Reliability of a Mechanized Procedure (Perkin-Elmer C4) for the Enzymatic Determination of... Introduction Kageyama reported recently (1) a new procedure for the direct enzymatic measurement of the uric acid concen* tiation in urine and serum. This method uses the Hmtzsch* reaction to determine formaldehyde formed from uric acid by means of the two enzymes unease (EC 1.7.3.3) and catalase (EC 1.11.1.6). The resulting color (3,5-diacetyl-l,4-dihydrolutidine) has its absorbance maximum at 410 nm. This procedure appeared to be suitable for the mechanic zation with a discrete analytical system. For this purpose we used the Perkin-Elmer C4 Automatic Analyzer, a combined-discrete system (2), since the reaction mixture is transported in single cups and then transferred into a discontinuous flow system for the photometric measurement. J. Clin. Chem. Clin. Biochem. / VoL 14,1976 / No. 4 In the following investigations on the reliability of the Kageyama method adjusted to the C4 analyzer are reported. The direct enzymatic UV-procedure ofPraetorius (3) was chosen as the reference and performed manually because we did not succeed in adapting this method to the C4 analyzer with sufficient precision. A sample blank assay treated with a glycerol solution instead of the unease suspension was used according to Kortum(4). Methods and Materials Operation of the C4 Automatic Analyzer The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

The Reliability of a Mechanized Procedure (Perkin-Elmer C4) for the Enzymatic Determination of Uric Acid According to Kageyama

Haeckel, R.
Clinical Chemistry and Laboratory Medicine , Volume 14 (1-12) – Jan 1, 1976
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References (8)

Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1976.14.1-12.165
Publisher site
See Article on Publisher Site

Abstract

Introduction Kageyama reported recently (1) a new procedure for the direct enzymatic measurement of the uric acid concen* tiation in urine and serum. This method uses the Hmtzsch* reaction to determine formaldehyde formed from uric acid by means of the two enzymes unease (EC 1.7.3.3) and catalase (EC 1.11.1.6). The resulting color (3,5-diacetyl-l,4-dihydrolutidine) has its absorbance maximum at 410 nm. This procedure appeared to be suitable for the mechanic zation with a discrete analytical system. For this purpose we used the Perkin-Elmer C4 Automatic Analyzer, a combined-discrete system (2), since the reaction mixture is transported in single cups and then transferred into a discontinuous flow system for the photometric measurement. J. Clin. Chem. Clin. Biochem. / VoL 14,1976 / No. 4 In the following investigations on the reliability of the Kageyama method adjusted to the C4 analyzer are reported. The direct enzymatic UV-procedure ofPraetorius (3) was chosen as the reference and performed manually because we did not succeed in adapting this method to the C4 analyzer with sufficient precision. A sample blank assay treated with a glycerol solution instead of the unease suspension was used according to Kortum(4). Methods and Materials Operation of the C4 Automatic Analyzer The

Journal

Clinical Chemistry and Laboratory Medicine – de Gruyter

Published: Jan 1, 1976

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