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Effect of Oxygen Shortage on the Metabolism of Oestrone in the Hemoglobin-free Perfused Rat Liver

Effect of Oxygen Shortage on the Metabolism of Oestrone in the Hemoglobin-free Perfused Rat Liver Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 1215 - 1221, September 1976 Effect of Oxygen Shortage on the Metabolism of Oestrone in the Hemoglobin-free Perfused Rat Liver* Michael HÖLLER, Claudia CHALYBÄUS** and Heinz BREUER Institut für Klinische Biochemie der Universität Bonn. (Received 23 February 1976) Summary: The isolated rat liver was perfused with a haemoglobin-free, albumin containing salt solution at 28° and 37 °C, respectively, with [4-14C]oestrone as substrate. During perfusion, the functional state of the liver was checked by continuously measuring the oxygen pressure and hydrogen ion concentration in the perfusion medium, flow rate, oxygen tension at various areas of the liver surface, and oxygen consumption; in addition, the following biochemical parameters were determined: ATP, ADP, lactate and pyruvate in liver tissue, and lactate and pyruvate in the perfusion medium. After 30 min of perfusion, free steroids, steroid glucuronides, steroid sulphates and the remaining water-soluble metabolites, present in liver tissue, perfusion medium and bile, were separated from each other and characterised by thin-layer and paper chromatography. It was found that, during perfusion at 37 °C, less hydroxylated metabolites were formed than at 28 °C. In contrast, metabolites whose formation is not directly oxygen-dependent, such as glucuronides and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Effect of Oxygen Shortage on the Metabolism of Oestrone in the Hemoglobin-free Perfused Rat Liver

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References (3)

Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.2.1215
Publisher site
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Abstract

Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 1215 - 1221, September 1976 Effect of Oxygen Shortage on the Metabolism of Oestrone in the Hemoglobin-free Perfused Rat Liver* Michael HÖLLER, Claudia CHALYBÄUS** and Heinz BREUER Institut für Klinische Biochemie der Universität Bonn. (Received 23 February 1976) Summary: The isolated rat liver was perfused with a haemoglobin-free, albumin containing salt solution at 28° and 37 °C, respectively, with [4-14C]oestrone as substrate. During perfusion, the functional state of the liver was checked by continuously measuring the oxygen pressure and hydrogen ion concentration in the perfusion medium, flow rate, oxygen tension at various areas of the liver surface, and oxygen consumption; in addition, the following biochemical parameters were determined: ATP, ADP, lactate and pyruvate in liver tissue, and lactate and pyruvate in the perfusion medium. After 30 min of perfusion, free steroids, steroid glucuronides, steroid sulphates and the remaining water-soluble metabolites, present in liver tissue, perfusion medium and bile, were separated from each other and characterised by thin-layer and paper chromatography. It was found that, during perfusion at 37 °C, less hydroxylated metabolites were formed than at 28 °C. In contrast, metabolites whose formation is not directly oxygen-dependent, such as glucuronides and

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hoppe-seyler's zeitschrift für physiologische chemie – de Gruyter

Published: Jan 1, 1976

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