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- Publisher
- de Gruyter
- Copyright
- Copyright © 2007 by the
- ISSN
- 14374315
- eISSN
- 14316730
- DOI
- 10.1515/BC.2007.065
- pmid
- 17570826
- Publisher site
- See Article on Publisher Site
Abstract
Abstract The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana . Kinetic analyses of tertiary stabilised ribozymes from peach latent mosaic viroid (PLMVd), Schistosoma mansoni and A. thaliana revealed a ten-fold difference in cleavage rates. Core nucleotide variations affected cleavage reactions least in the A. thaliana ribozyme, and most in the S. mansoni ribozyme. The reverse ligation reaction was catalysed efficiently by the PLMVd and A. thaliana ribozymes. The different behaviour of the individual hammerhead ribozymes is discussed in terms of structure and function.
Journal
Biological Chemistry – de Gruyter
Published: Jul 1, 2007