Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland
Abstract
Multi-locus sequencing typing (MLST) is widely used to monitor the phylogeny of microbial OPENACCESS outbreaks. However, several strains of vancomycin-resistant Enterococcus faecium Citation: Lemonidis K, Salih TS, Dancer SJ, Hunter (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few IS, Tucker NP (2019) Emergence of an Australian- cases also reported in England. Here, we identified similarly distinct strains circulating in two like pstS-null vancomycin resistant Enterococcus neighbouring hospitals in Scotland. Whole genome sequencing of five VREfm strains iso- faecium clone in Scotland. PLoS ONE 14(6): e0218185. https://doi.org/10.1371/journal. lated from these hospitals identified four pstS-null strains in both hospitals, while the fifth pone.0218185 was multi-locus sequence type (ST) 262, which is the first documented in the UK. All five Editor: Yung-Fu Chang, Cornell University, UNITED Scottish isolates had an insertion in the tetM gene, which is associated with increased sus- STATES ceptibility to tetracyclines, providing no other tetracycline-resistant gene is present. Such an Received: January 3, 2019 insertion, which encompasses a dfrG gene and two currently uncharacterised genes, was additionally identified in all tested vanA-type pstS-null VREfm strains (5 English and 68 Aus- Accepted: May 28, 2019 tralian). Phylogenetic comparison with other VREfm genomes
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