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A Method for Age Synchrony of Panagrellus Redivivus in Xenic Culture

A Method for Age Synchrony of Panagrellus Redivivus in Xenic Culture A METHOD FOR AGE SYNCHRONY OF PANAGRELLUS REDIVIVUS IN XENIC CULTURE BY JOHN A. BOLLINGER and JAMES D. WILLETT The Department of Chemistry, University of Idaho, Moscow, Idaho, U.S.A. A method for synchronizing yeast fed populations of female Panagrellus redivivus has been developed. Synchronous cultures are begun with 1 or 2 day old larvae and resynchronized at 7 days of age and every 3 days thereafter. Synchrony is obtained by separating parents from progeny, and females from males, on sucrose gradients. Using this method synchrony has been maintained from the peak of reproduction to the median life span of individuals in selected populations. Methods are available for isolating larval and young adult nematodes from asyn- chronous populations (Chow & Pasternak, 1969; Sulston & Brenner, 1974; Hieb & Rothstein, 1975). No suitable method exists for obtaining sufficient numbers of older nematodes for chemical analyses (Zuckerman, 1976). The problem has been to eliminate progeny from adult populations. We have attempted to solve this problem by intermittent selection synchrony. The adults are continuously cultured and populations of old individuals of the same age, length, and sex are obtained. MATERIALS AND METHODS Yeast extract, malt extract, peptone, soy peptone, bacto-liver, beef-heart infusion http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

A Method for Age Synchrony of Panagrellus Redivivus in Xenic Culture

Bollinger, John A.; Willett, James D.
Nematologica , Volume 24 (4): 6 – Jan 1, 1978
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529278X00524
Publisher site
See Article on Publisher Site

Abstract

A METHOD FOR AGE SYNCHRONY OF PANAGRELLUS REDIVIVUS IN XENIC CULTURE BY JOHN A. BOLLINGER and JAMES D. WILLETT The Department of Chemistry, University of Idaho, Moscow, Idaho, U.S.A. A method for synchronizing yeast fed populations of female Panagrellus redivivus has been developed. Synchronous cultures are begun with 1 or 2 day old larvae and resynchronized at 7 days of age and every 3 days thereafter. Synchrony is obtained by separating parents from progeny, and females from males, on sucrose gradients. Using this method synchrony has been maintained from the peak of reproduction to the median life span of individuals in selected populations. Methods are available for isolating larval and young adult nematodes from asyn- chronous populations (Chow & Pasternak, 1969; Sulston & Brenner, 1974; Hieb & Rothstein, 1975). No suitable method exists for obtaining sufficient numbers of older nematodes for chemical analyses (Zuckerman, 1976). The problem has been to eliminate progeny from adult populations. We have attempted to solve this problem by intermittent selection synchrony. The adults are continuously cultured and populations of old individuals of the same age, length, and sex are obtained. MATERIALS AND METHODS Yeast extract, malt extract, peptone, soy peptone, bacto-liver, beef-heart infusion

Journal

Nematologica – Brill

Published: Jan 1, 1978

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