Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

: The authority of the subfamily name Heteroderinae

: The authority of the subfamily name Heteroderinae 563 tions as a reference, has a refractive index of 1.455. The authors modified routine embedding for electron microscopy to give a suitable method for preparing mounts for light microscopy. Nematodes are picked out from samples by conventional methods, cooled to 4°, assembled in a double sieve (De Grisse, 1969), fixed for at least 2 hr in 4 % paraldehyde in Sorensenbuffer at 4°, rinsed in buffer, post- fixed in 1 % osmic acid in buffer for 1-2 hr, dehydrated in acetone by Sitte's (1962) pressure method, transferred to a 1:4 mixture of ERL (Spurr, 1969) and acetone, in which they are left for at least 20 hr, then transferred to 100 % ERL for 2 hr. The sieve is opened and the nematodes are placed in a drop of ERL on a conventional slide, covered with a coverslip which will adjust itself correctly without flattening the nematodes. The slides are exposed to 60° for at least 24 hr. If further investigations of the specimen are desired, the slide is placed in a petri dish filled with propylene oxide for ca. 24 hr, after which the coverslip will have separated from the resin plate. The latter is re-embedded http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

: The authority of the subfamily name Heteroderinae

Wouts, W.M.; Weischer, B.
Nematologica , Volume 19 (4): 3 – Jan 1, 1973
Download PDF
3 pages

Loading next page...
 
/lp/brill/the-authority-of-the-subfamily-name-heteroderinae-v3oTkRl3KX

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529273X00600
Publisher site
See Article on Publisher Site

Abstract

563 tions as a reference, has a refractive index of 1.455. The authors modified routine embedding for electron microscopy to give a suitable method for preparing mounts for light microscopy. Nematodes are picked out from samples by conventional methods, cooled to 4°, assembled in a double sieve (De Grisse, 1969), fixed for at least 2 hr in 4 % paraldehyde in Sorensenbuffer at 4°, rinsed in buffer, post- fixed in 1 % osmic acid in buffer for 1-2 hr, dehydrated in acetone by Sitte's (1962) pressure method, transferred to a 1:4 mixture of ERL (Spurr, 1969) and acetone, in which they are left for at least 20 hr, then transferred to 100 % ERL for 2 hr. The sieve is opened and the nematodes are placed in a drop of ERL on a conventional slide, covered with a coverslip which will adjust itself correctly without flattening the nematodes. The slides are exposed to 60° for at least 24 hr. If further investigations of the specimen are desired, the slide is placed in a petri dish filled with propylene oxide for ca. 24 hr, after which the coverslip will have separated from the resin plate. The latter is re-embedded

Journal

Nematologica – Brill

Published: Jan 1, 1973

There are no references for this article.