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118 reproduction of this free-living nematode; furthermore, most of them appear to be of the type described by the authors. For these reasons, chemical mutagens have been used, the most efficient being E.M.S. The best yield of mutants is obtained when 2.5-day-old larvae, with undeveloped gonads, are immersed in a medium made of I:.M.S, dissolved in saline solution (1.8 NaCl/1) at 18° C. Mutants have been produced by concentrations of E.M.S. varying from 0.06% to 0.5%. At high concentrations of E.M.S., the immersion time required is from 1 to 5 hours; at low concentrations, from 20 to 24 hours. The larvae are afterwards transferred on drops of nutrient agar medium inoculated with bacterial culture. The 150 individuals, treated as above, produced an offspring of about 10,000 animals. Among this F1 progeny, ten variants showed cuticle swellings and size modifications (dwarfness or giant- ness). Most of them - and in any case all the giants - were sterile; the others gave a normal progeny by self-fertilization (= phenocopy effect). Among the rest of the Fl progeny, which looked normal, 150 adults were randomly chosen and transferred in individual culture in order to get the Fz generation. Of these F2
Nematologica – Brill
Published: Jan 1, 1973
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