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- Publisher
- Brill
- Copyright
- Copyright © Koninklijke Brill NV, Leiden, The Netherlands
- ISSN
- 0028-2596
- eISSN
- 1875-2926
- DOI
- 10.1163/187529267X00751
- Publisher site
- See Article on Publisher Site
Abstract
472 JONES, F. G. W., & PAWELSKA, K. (1963). The behaviour of populations of potato root-eelworm (Heterodera rostochiensis Woll.) towards some resistant tuberous and other Solanum species. Ann. appl. Biol. 51, 277-294. SHORT COMMUNICATIONS O. M. BROOKS 1): Polystyrene beakers for eelworm culture. For screening numbers of different eelwoim populations against resistant potato seedlings to determine pathotype, JONES (1957) recommends the use of 9 cm diameter clay pots. The disadvan- tages of using such pots in these experiments are the time taken to tip out the plants for frequent inspection, the risk of cross contamination, the disruption of the root system and possible pot breakage. To overcome these disadvantages we have substituted polystyrene beakers in large scale tests for potato cyst eelworm (Heterodera ro.rtochTen.rz.r, Woll.) pathotype determinations. The beakers are 7.5 cm diameter at top, tapering to 5.5 cm at base, and 10.5 cm high and were obtained from Pioneer Plastic Containers Ltd., Great South West Road, Bedfont, Feltham, Middlesex at a cost of £2-2-0 for 144. Three drainage holes, each of about 8 mm diameter were melted through the base with a small electric soldering iron. These beakers are virtually unbreakable and the complete root mat surface may be examined through the transparent wall without disruption. Cysts were clearly visible without disturbance of the roots and the developing eelworms could be observed by placing the beaker on its side under a binocular microscope. In our experiments the beakers were stood out in a wet peat plunge to prevent scratching of the polystyrene surfaces, the development of algae, and to assist in keeping the soil ball moist. The pots remained clear throughout the season, after which they were easily rinsed clean and stored in good condition for further use. JONES, F. G. W. (1957). Resistance breaking biotypes of the potato root eelworm (Heterodera rostochiensis Woll.), Nematologica 2, 185-192. 1) National Agricultural Advisory Service, Shardlow Hall, Shardlow, Derby, Gr. Britain. N. A. CROLL 1): A 'Y' shaped trough for use in nematode behavioural studies. Studies on nematode orientation have been either by direct observation or by indirectly assessing the distribution of eelworms after unit time in a physical or chemical gradient. In this way the nematodes have only been given the choice of responding positively or negatively. If their real response is to move tangentially out of the gradient, experimental design has not usually allowed for this alternative. To introduce a second element of choice, i.e. to respond or to avoid the classical 'Y' tube has been adapted for investigating nematode behaviour. A trough is ground from a perspex block (Fig. 1); it has a 'Y' shaped channel with a flat bottom and sloping sides (to avoid refraction when observed through a microscope). The angle subtended at the base of the branch is equal on both sides (x). At the tips of the arms are two wells into which the eelworms drop; this has been found very useful for all but the most active nematodes, for once they have fallen into the well they cannot swim out. After a given time the number in each well is compared. This technique is at present being employed to investigate the galvanotactic response of Panagrellus redivivus. 1) Imperial College Field Station, Ashurst Lodge, Sunninghill, Ascot, Berkshire, England.
Journal
Nematologica – Brill
Published: Jan 1, 1967