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Tissue Culture and Maintenance of the Root-Lesion Nematode, Pratylenchus Vulnus

Tissue Culture and Maintenance of the Root-Lesion Nematode, Pratylenchus Vulnus TISSUE CULTURE AND MAINTENANCE OF THE ROOT-LESION NEMATODE, PRATYLENCHUS VULNUS BY B. F. LOWNSBERY, C. S. HUANG, and RUTH N. JOHNSON Department of Nematology, University of California, Davis, U.S.A. A mixture of callus and differentiated alfalfa tissue grown on a nutrient agar provides a favorable medium for the monoxenic culture of Pratylenchus vulnus. The nematode increases more rapidly at 25° C than at 20°, and does not increase at 30° or 35°. At 25°, high populations of P. vulnus can be obtained 2 months after inoculation of the cultures. These high populations can be maintained longer at 15° or 10° than at 5°, 20°, or 25°. Nematode-free alfalfa tissue grew well over the range 20° to 30°, poorly at 35°. P. vulnus reduced the fresh weight of alfalfa callus at 20° and 25°, the temperatures favorable for nematode repro- duction. Pratylenchu.r vulnu.r Allen & Jensen is the most widely distributed root-lesion nematode in deciduous fruit orchards in California. Studies of the pathogenicity of P. vulnu.r to deciduous fruit rootstocks require large numbers of the nematode. These can be reared on bush beans (Phaseolus vulgaris L. ) in a glasshouse tank of soil infested with P. vulnu.r, and extracted http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Tissue Culture and Maintenance of the Root-Lesion Nematode, Pratylenchus Vulnus

Lownsbery, B.F.; Huang, C.S.; Johnson, Ruth N.
Nematologica , Volume 13 (3): 5 – Jan 1, 1967
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529267X00625
Publisher site
See Article on Publisher Site

Abstract

TISSUE CULTURE AND MAINTENANCE OF THE ROOT-LESION NEMATODE, PRATYLENCHUS VULNUS BY B. F. LOWNSBERY, C. S. HUANG, and RUTH N. JOHNSON Department of Nematology, University of California, Davis, U.S.A. A mixture of callus and differentiated alfalfa tissue grown on a nutrient agar provides a favorable medium for the monoxenic culture of Pratylenchus vulnus. The nematode increases more rapidly at 25° C than at 20°, and does not increase at 30° or 35°. At 25°, high populations of P. vulnus can be obtained 2 months after inoculation of the cultures. These high populations can be maintained longer at 15° or 10° than at 5°, 20°, or 25°. Nematode-free alfalfa tissue grew well over the range 20° to 30°, poorly at 35°. P. vulnus reduced the fresh weight of alfalfa callus at 20° and 25°, the temperatures favorable for nematode repro- duction. Pratylenchu.r vulnu.r Allen & Jensen is the most widely distributed root-lesion nematode in deciduous fruit orchards in California. Studies of the pathogenicity of P. vulnu.r to deciduous fruit rootstocks require large numbers of the nematode. These can be reared on bush beans (Phaseolus vulgaris L. ) in a glasshouse tank of soil infested with P. vulnu.r, and extracted

Journal

Nematologica – Brill

Published: Jan 1, 1967

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