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PREPARATION OF FRESH FROZEN SECTIONS OF FREE-LIVING AND PLANT PARASITIC NEMATODES WITH A COLD MICROTOME BY KARL H. DEUBERT and BERT M. ZUCKERMAN University of Massachusetts, Cranberry Experiment Station, East Wareham, Mass., U.S.A. A technique is described for the preparation of fresh frozen sections for histochemical studies. This technique fulfills most requirements for histochemical work, and the quality of the sections is satisfactory. Paraffin sections are not always suitable for'the histochemical detection especially of enzymes, and frozen sections of fixed or unfixed tissues must be prepared. The preparation of frozen sections of nematodes, however, poses technical diffi- culties because of the small size of the objects. The current paper describes a method to obtain fresh frozen sections suitable for the detection of enzymes. Fresh frozen sections of nematodes are prepared with a rotary microtome (Lip- shaw Standard Model 50-AB) mounted in a cryostat (Lipshaw Cryotome Model 1500, Lipshaw Manufacturing Co., Detroit, Mich.). Before cooling the microtome, it is advisable to remove the thermometer from its holder on the back-wall of the freezing chamber, and bend it towards the microtome. Temperature readings thus obtained come closer to the temperature of the microtome than with the thermometer in its original
Nematologica – Brill
Published: Jan 1, 1967
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