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Preparation of Fresh Frozen Sections of Free-Living and Plant Parasitic Nematodes With a Cold Microtome

Preparation of Fresh Frozen Sections of Free-Living and Plant Parasitic Nematodes With a Cold... PREPARATION OF FRESH FROZEN SECTIONS OF FREE-LIVING AND PLANT PARASITIC NEMATODES WITH A COLD MICROTOME BY KARL H. DEUBERT and BERT M. ZUCKERMAN University of Massachusetts, Cranberry Experiment Station, East Wareham, Mass., U.S.A. A technique is described for the preparation of fresh frozen sections for histochemical studies. This technique fulfills most requirements for histochemical work, and the quality of the sections is satisfactory. Paraffin sections are not always suitable for'the histochemical detection especially of enzymes, and frozen sections of fixed or unfixed tissues must be prepared. The preparation of frozen sections of nematodes, however, poses technical diffi- culties because of the small size of the objects. The current paper describes a method to obtain fresh frozen sections suitable for the detection of enzymes. Fresh frozen sections of nematodes are prepared with a rotary microtome (Lip- shaw Standard Model 50-AB) mounted in a cryostat (Lipshaw Cryotome Model 1500, Lipshaw Manufacturing Co., Detroit, Mich.). Before cooling the microtome, it is advisable to remove the thermometer from its holder on the back-wall of the freezing chamber, and bend it towards the microtome. Temperature readings thus obtained come closer to the temperature of the microtome than with the thermometer in its original http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Preparation of Fresh Frozen Sections of Free-Living and Plant Parasitic Nematodes With a Cold Microtome

Nematologica , Volume 13 (3): 4 – Jan 1, 1967

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529267X00562
Publisher site
See Article on Publisher Site

Abstract

PREPARATION OF FRESH FROZEN SECTIONS OF FREE-LIVING AND PLANT PARASITIC NEMATODES WITH A COLD MICROTOME BY KARL H. DEUBERT and BERT M. ZUCKERMAN University of Massachusetts, Cranberry Experiment Station, East Wareham, Mass., U.S.A. A technique is described for the preparation of fresh frozen sections for histochemical studies. This technique fulfills most requirements for histochemical work, and the quality of the sections is satisfactory. Paraffin sections are not always suitable for'the histochemical detection especially of enzymes, and frozen sections of fixed or unfixed tissues must be prepared. The preparation of frozen sections of nematodes, however, poses technical diffi- culties because of the small size of the objects. The current paper describes a method to obtain fresh frozen sections suitable for the detection of enzymes. Fresh frozen sections of nematodes are prepared with a rotary microtome (Lip- shaw Standard Model 50-AB) mounted in a cryostat (Lipshaw Cryotome Model 1500, Lipshaw Manufacturing Co., Detroit, Mich.). Before cooling the microtome, it is advisable to remove the thermometer from its holder on the back-wall of the freezing chamber, and bend it towards the microtome. Temperature readings thus obtained come closer to the temperature of the microtome than with the thermometer in its original

Journal

NematologicaBrill

Published: Jan 1, 1967

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