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ATTEMPTS TO INOCULATE XIPHINEMA INDEX WITH GRAPE FANLEAF VIRUS BY MICROINOCULATION BY E. BETTO *) AND D. J. RASKI Department of Nematology, University of California, Davis, U.S.A. Techniques and microequipment to mechanically inoculate X, index with grape fanleaf virus are described. There was no evidence from mechanical inoculation tests on Chenopodium quinoa that the virus was introduced into adult female X. index by microinoculation, although the nematodes survived the injury caused by microneedles, later fed on grape seedlings, and apparently reproduced normally. Since the research by Hewitt, Raski & Goheen (1958) implicated nematodes as vectors of plant viruses and proved the dagger nematode, Xiphinema index Thorne & Allen, to be the vector of soil-borne grapevine fanleaf virus (GFV), increased attention had been paid to virus-vector relationship (as summarized by Raski & Hewitt, 1963). If techniques for mechanical transmission of virus to its nematode vector were available, the exact timing of virus acquisition, the incubation period in the vector and whether the virus multiplies in the nematode could be determined. This report presents our attempts to transmit GFV to its vector, X. index, mechanically. ' MATERIALS AND METHODS Virus-free X. index were reared and maintained in the greenhouse on
Nematologica – Brill
Published: Jan 1, 1966
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