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(1953)
A colorimetric determination of number
F. Jones (1954)
FIRST STEPS IN BREEDING FOR RESISTANCE TO POTATO‐ROOT EELWORM.Annals of Applied Biology, 41
C. Ellenby, A. Gilbert (1960)
A Convenient Modification of the Dilution Hatching Trial for the Potato-root Eelworm, Heterodera rostochiensis WollenweberJournal of Helminthology, 34
D. Fenwick, E. Reid (1951)
The Use of a Microbalance in Putting up Uniformly Sized Batches of Heterodera Cysts for Experiment.Journal of Helminthology, 25
On the effect of root diffusates from Tagetes spp . on Heterodera rosto - chiensis Woll
C. Blake (1959)
A turbidimetric method for estimating the number of nematode larvae in a suspension., 83
(1962)
Emergence of larvae from cysts of the genus Heterodera
K. Carroll (1958)
Purification and Properties of Eelworm Hatching FactorsNematologica, 3
D. Fenwick, E. Widdowson (1958)
The Conduct of Hatching Tests on Cysts of the Potato-root Eelworm Heterodera rostochiensis (Woll.)Journal of Helminthology, 32
K. Carroll, A. Todd, A. Johnson, J. Heyes (1958)
The Potato Eelworm Hatching Factor 7. Further Methods for Concentration of the FactorNematologica, 3
D. Fenwick (1952)
THE BIO‐ASSAY OF POTATO‐ROOT DIFFUSATEAnnals of Applied Biology, 39
A. Shepherd (1962)
New Blue R, a Stain That Differentiates Between Living and Dead NematodesNematologica, 8
(1961)
On the effect of root diffusates from Tagetes spp
E. Widdowson, G. Wiltshire (1958)
THE POTATO‐EELWORM HATCHING FACTORAnnals of Applied Biology, 46
A. Lovett (1917)
The Potato Eelworm
(1960)
Biochemistry department
(1958)
Purification and properties of eelworm hatching
A BIOASSAY FOR HETERODERA SPP. WITHOUT COUNTING CYSTS OR LARVAE BY F. G. W. JONES & MARGARET C. GANDER Rothamsted Experimental Station, Harpenden, England Cyst batches of approximately equal size for use in bioassays can be set up with a simple pipette and the larvae that hatch from them can be assessed by absorptiometry, which saves the time and tedium of counting both cysts and larvae. In an experiment on the use of charcoal to recover the hatching factor from potato root diffusate, the results of bioassays by this method agreed well with those based on counts. The interpretation of data is discussed. The hatching test devised by Fenwick & Widowson ( 1958) for cysts of Hete- rodera rostochiensis W. has been used routinely for over 10 years at Rothamsted and elsewhere in work on the hatching factor by Todd and his collaborators (see Carroll, Heyes, Johnson & Todd 1958), Widdowson & Wiltshire (1958), Clarke ( 1960) and has been modified for use with other Heterodera spp. by Winslow (see Carroll 1 9 5 8 ) . Essentially the method consists of plying replicated batches of 100 or more cysts with dilutions of test solutions and comparing the
Nematologica – Brill
Published: Jan 1, 1962
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