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A Bioassay for Heterodera Spp. Without Counting Cysts or Larvae

A Bioassay for Heterodera Spp. Without Counting Cysts or Larvae A BIOASSAY FOR HETERODERA SPP. WITHOUT COUNTING CYSTS OR LARVAE BY F. G. W. JONES & MARGARET C. GANDER Rothamsted Experimental Station, Harpenden, England Cyst batches of approximately equal size for use in bioassays can be set up with a simple pipette and the larvae that hatch from them can be assessed by absorptiometry, which saves the time and tedium of counting both cysts and larvae. In an experiment on the use of charcoal to recover the hatching factor from potato root diffusate, the results of bioassays by this method agreed well with those based on counts. The interpretation of data is discussed. The hatching test devised by Fenwick & Widowson ( 1958) for cysts of Hete- rodera rostochiensis W. has been used routinely for over 10 years at Rothamsted and elsewhere in work on the hatching factor by Todd and his collaborators (see Carroll, Heyes, Johnson & Todd 1958), Widdowson & Wiltshire (1958), Clarke ( 1960) and has been modified for use with other Heterodera spp. by Winslow (see Carroll 1 9 5 8 ) . Essentially the method consists of plying replicated batches of 100 or more cysts with dilutions of test solutions and comparing the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

A Bioassay for Heterodera Spp. Without Counting Cysts or Larvae

Nematologica , Volume 8 (1): 12 – Jan 1, 1962

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References (17)

Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529262X01007
Publisher site
See Article on Publisher Site

Abstract

A BIOASSAY FOR HETERODERA SPP. WITHOUT COUNTING CYSTS OR LARVAE BY F. G. W. JONES & MARGARET C. GANDER Rothamsted Experimental Station, Harpenden, England Cyst batches of approximately equal size for use in bioassays can be set up with a simple pipette and the larvae that hatch from them can be assessed by absorptiometry, which saves the time and tedium of counting both cysts and larvae. In an experiment on the use of charcoal to recover the hatching factor from potato root diffusate, the results of bioassays by this method agreed well with those based on counts. The interpretation of data is discussed. The hatching test devised by Fenwick & Widowson ( 1958) for cysts of Hete- rodera rostochiensis W. has been used routinely for over 10 years at Rothamsted and elsewhere in work on the hatching factor by Todd and his collaborators (see Carroll, Heyes, Johnson & Todd 1958), Widdowson & Wiltshire (1958), Clarke ( 1960) and has been modified for use with other Heterodera spp. by Winslow (see Carroll 1 9 5 8 ) . Essentially the method consists of plying replicated batches of 100 or more cysts with dilutions of test solutions and comparing the

Journal

NematologicaBrill

Published: Jan 1, 1962

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