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Recovery of Nematodes From Infected Roots By Maceration

Recovery of Nematodes From Infected Roots By Maceration RECOVERY OF NEMATODES FROM INFECTED ROOTS BY MACERATION BY VICTOR H. DROPKIN, WILSON L. SMITH Jr. and RONALD F. MYERS Crops Research Division, Agricultural Research Service, and Market Quality Research Division, Agricultural Marketing Service, United States Department of Agriculture, Beltsville, Maryland, U.S.A., respectively. Animal parasitologists have used enzyme digestion to separate nematodes from mammalian tissues, but so far as we know, this method has not been applied to nematodes parasitic in plants. Because various enzyme preparations and chemical methods are available to separate cells of plants, we undertook a survey of mace- ration procedures for the recovery of root-knot nematodes (Meloidogyne spp.) from root tissues. These included use of fluid from potato slices inoculated with a soft-rot bacterium, Erwinia carotovora ( L. R. Jones) Holland, commercial en- zyme preparations, HCI, periodic acid, and Jeffrey's macerating fluid. Infected root samples were exposed to enzyme or chemical activity in Erlen- meyer flasks under constant agitation by a wrist-action mechanical shaker. The softened tissues were then beaten for about 30 minutes in a blendor with its knives replaced by rubber flails. The speed of the blendor was controlled by a variable resistance. The resulting mixture was washed with tap water through http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Recovery of Nematodes From Infected Roots By Maceration

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529260X00082
Publisher site
See Article on Publisher Site

Abstract

RECOVERY OF NEMATODES FROM INFECTED ROOTS BY MACERATION BY VICTOR H. DROPKIN, WILSON L. SMITH Jr. and RONALD F. MYERS Crops Research Division, Agricultural Research Service, and Market Quality Research Division, Agricultural Marketing Service, United States Department of Agriculture, Beltsville, Maryland, U.S.A., respectively. Animal parasitologists have used enzyme digestion to separate nematodes from mammalian tissues, but so far as we know, this method has not been applied to nematodes parasitic in plants. Because various enzyme preparations and chemical methods are available to separate cells of plants, we undertook a survey of mace- ration procedures for the recovery of root-knot nematodes (Meloidogyne spp.) from root tissues. These included use of fluid from potato slices inoculated with a soft-rot bacterium, Erwinia carotovora ( L. R. Jones) Holland, commercial en- zyme preparations, HCI, periodic acid, and Jeffrey's macerating fluid. Infected root samples were exposed to enzyme or chemical activity in Erlen- meyer flasks under constant agitation by a wrist-action mechanical shaker. The softened tissues were then beaten for about 30 minutes in a blendor with its knives replaced by rubber flails. The speed of the blendor was controlled by a variable resistance. The resulting mixture was washed with tap water through

Journal

Nematologica – Brill

Published: Jan 1, 1960

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