Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Single-tube multiplex PCR and capillary electrophoresis for the detection of bcl2–IgH chimeras

Single-tube multiplex PCR and capillary electrophoresis for the detection of bcl2–IgH chimeras Haematologia , Vol. 31, No. 4, pp. 313– 317 (2002) Ó VSP 2002. Article Single-tube multiplex PCR and capillary electrophoresis for the detection of bcl2– IgH chimeras PIROSKA PÁLDI-HARIS 1 , GÁBOR RÁCZ 1 , KRISZTINA NÉMETH 2 and JÁNOS FÖLDI 1 ; ¤ 1 National Institute of Haematology and Immunology, Budapest, Hungary 2 Semmelweis Medical University, 2nd Pediatric Clinic, Budapest, Hungary Abstract —The molecular background of follicular lymphoma is the formation of chimera(s) by the bcl2 gene and one of the immunoglobulin genes, mainly the heavy chain gene. While the diagnosis of follicular lymphoma is based on histological study, the histology cannot be recommended for the follow-up of efŽ ciency of treatment(s). Sensitive detection of the chimera genes in blood or bone marrow samples can serve as informative data without invasive sampling. In this study, a single- tube multiplex reaction was developed for the simultaneous detection of different chimeras of the bcl2 and immunoglobulin heavy chain genes, molecular hallmarks of follicular lymphoma. The method was optimized for the highest yield of reaction and for reproducing the ratio of components in artiŽ cial mixtures of two different chimeras. The method is suitable for fast and accurate molecular characterization http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Haematologia Brill

Single-tube multiplex PCR and capillary electrophoresis for the detection of bcl2–IgH chimeras

Loading next page...
 
/lp/brill/single-tube-multiplex-pcr-and-capillary-electrophoresis-for-the-e8YkwWLr6T

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Brill
Copyright
© 2001 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0017-6559
eISSN
1568-5594
DOI
10.1163/15685590160141332
Publisher site
See Article on Publisher Site

Abstract

Haematologia , Vol. 31, No. 4, pp. 313– 317 (2002) Ó VSP 2002. Article Single-tube multiplex PCR and capillary electrophoresis for the detection of bcl2– IgH chimeras PIROSKA PÁLDI-HARIS 1 , GÁBOR RÁCZ 1 , KRISZTINA NÉMETH 2 and JÁNOS FÖLDI 1 ; ¤ 1 National Institute of Haematology and Immunology, Budapest, Hungary 2 Semmelweis Medical University, 2nd Pediatric Clinic, Budapest, Hungary Abstract —The molecular background of follicular lymphoma is the formation of chimera(s) by the bcl2 gene and one of the immunoglobulin genes, mainly the heavy chain gene. While the diagnosis of follicular lymphoma is based on histological study, the histology cannot be recommended for the follow-up of efŽ ciency of treatment(s). Sensitive detection of the chimera genes in blood or bone marrow samples can serve as informative data without invasive sampling. In this study, a single- tube multiplex reaction was developed for the simultaneous detection of different chimeras of the bcl2 and immunoglobulin heavy chain genes, molecular hallmarks of follicular lymphoma. The method was optimized for the highest yield of reaction and for reproducing the ratio of components in artiŽ cial mixtures of two different chimeras. The method is suitable for fast and accurate molecular characterization

Journal

HaematologiaBrill

Published: Jan 1, 2001

Keywords: follicular lymphoma; Multiplex PCR

There are no references for this article.