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(2008)
A phylogenetic framework for root-lesion nematodes of the genus 437
T. Uehara, A. Kushida, Y. Momota (2001)
PCR-based cloning of two Ξ-1,4-endoglucanases from the root-lesion nematode Pratylenchus penetransNematology, 3
E. Fanelli, A. Troccoli, Francesco Capriglia, G. Lucarelli, N. Vovlas, N. Greco, F. Luca (2018)
Sequence variation in ribosomal DNA and in the nuclear hsp90 gene of Pratylenchus penetrans (Nematoda: Pratylenchidae) populations and phylogenetic analysisEuropean Journal of Plant Pathology, 152
Diagnose de fitonematoides
F. Luca, A. Reyes, A. Troccoli, P. Castillo (2011)
Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS rDNAEuropean Journal of Plant Pathology, 130
(2012)
Detection and quantification of Pratylenchus 468 thornei in DNA extracted from soil using real - time PCR
L. Al-Banna, Valerie Williamson, S. Gardner (1997)
Phylogenetic analysis of nematodes of the genus Pratylenchus using nuclear 26S rDNA.Molecular phylogenetics and evolution, 7 1
P. Castillo, N. Vovlas (2008)
Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management
Pratylenchus (Nematoda): evidence from 18S and D2-D3 expansion segments of 28S 438 ribosomal RNA genes and morphological characters
S. Subbotin, Erik Ragsdale, T. Mullens, P. Roberts, M. Mundo‐Ocampo, J. Baldwin (2008)
A phylogenetic framework for root lesion nematodes of the genus Pratylenchus (Nematoda): Evidence from 18S and D2-D3 expansion segments of 28S ribosomal RNA genes and morphological characters.Molecular phylogenetics and evolution, 48 2
John Jones, A. Haegeman, E. Danchin, H. Gaur, J. Helder, Michael Jones, T. Kikuchi, R. Manzanilla-López, J. Palomares-Rius, W. Wesemael, R. Perry (2013)
Top 10 plant-parasitic nematodes in molecular plant pathology.Molecular plant pathology, 14 9
(2016)
Species - specific diagnostics using a β1 , 4 - endoglucanase 417 gene for Pratylenchus spp
Johan Hoogen, S. Geisen, D. Routh, H. Ferris, W. Traunspurger, D. Wardle, R. Goede, Byron Adams, W. Ahmad, W. Andriuzzi, R. Bardgett, M. Bonkowski, R. Campos-Herrera, J. Cares, T. Caruso, Larissa Caixeta, Xiaoyun Chen, Sofia Costa, R. Creamer, José Castro, M. Dam, D. Djigal, M. Escuer, Bryan Griffiths, C. Gutiérrez, Karin Hohberg, D. Kalinkina, P. Kardol, Alan Kergunteuil, G. Korthals, Valentyna Krashevska, A. Kudrin, Qi Li, W. Liang, Matthew Magilton, M. Marais, José Martín, E. Matveeva, E. Mayad, C. Mulder, P. Mullin, R. Neilson, T. Nguyen, Uffe Nielsen, H. Okada, Juan Rius, K. Pan, V. Peneva, L. Pellissier, Júlio Silva, Camille Pitteloud, Thomas Powers, K. Powers, C. Quist, S. Rasmann, S. Moreno, S. Scheu, H. Setälä, A. Sushchuk, A. Tiunov, J. Trap, W. Putten, Mette Vestergård, C. Villenave, L. Waeyenberge, D. Wall, R. Wilschut, Daniel Wright, Jiue-in Yang, T. Crowther (2019)
Soil nematode abundance and functional group composition at a global scaleNature, 572
(1998)
Non-manual lysis of second-stage 432
(2019)
New insights into 459 nematode DNA - metabarcoding as revealed by the characterization of artificial and spiked 460 nematode communities
E. Fanelli, A. Troccoli, E. Picardi, C. Pousis, F. Luca (2014)
Molecular characterization and functional analysis of four β‐1,4‐endoglucanases from the root‐lesion nematode Pratylenchus vulnusPlant Pathology, 63
(2016)
Diagnose molecular de 422 nematoides parasitos de plantas . Molecular diagnostics of plant - parasitic nematodes
F. Mokrini, L. Waeyenberge, N. Viaene, Fouad Andaloussi, M. Moens (2013)
Quantitative detection of the root-lesion nematode, Pratylenchus penetrans, using qPCREuropean Journal of Plant Pathology, 137
T. Uehara, T. Mizukubo, A. Kushida, Y. Momota (1998)
Identification of Pratylenchus penetrans (COBB) by PCR Using ITS-Based Species-Specific PrimersJapanese Journal of Nematology, 28
D. Jesus, C. Oliveira, D. Roberts, V. Blok, R. Neilson, T. Prior, H. Balbino, K. MacKenzie, R. Oliveira (2016)
Morphological and molecular characterisation of Aphelenchoides besseyi and A. fujianensis (Nematoda: Aphelenchoididae) from rice and forage grass seeds in BrazilNematology, 18
S. Donn, B. Griffiths, R. Neilson, T. Daniell (2008)
DNA extraction from soil nematodes for multi-sample community studiesApplied Soil Ecology, 38
T. Janssen, G. Karssen, V. Orlando, S. Subbotin, W. Bert (2017)
Molecular characterization and species delimiting of plant-parasitic nematodes of the genus Pratylenchus from the penetrans group (Nematoda: Pratylenchidae).Molecular phylogenetics and evolution, 117
(2003)
Molecular identification of cystforming 441 nematodes ( Heteroderidae ) from Iran and a phylogeny based on the ITS sequences of 442 rDNA
S. T., Harris, J. L., Sandall (2019)
Identification of Single Identification of Single MeloidogyneMeloidogyne Juveniles by Polymerase Juveniles by Polymerase Chain Reaction Amplification of Mitochondrial DNA Chain Reaction Amplification of Mitochondrial DNA
(2019)
Technical and economic efficiency of methods for extracting genomic DNA 343 from Meloidogyne javanica
C. Schrader, A. Schielke, L. Ellerbroek, R. Johne (2012)
PCR inhibitors – occurrence, properties and removalJournal of Applied Microbiology, 113
J. Stanton, C. McNicol, V. Steele (1998)
Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reactionAustralasian Plant Pathology, 27
T. Powers, T. Harris (1993)
A polymerase chain reaction method for identification of five major meloidogyne species.Journal of nematology, 25 1
A. Peetz, I. Zasada (2016)
Species-specific diagnostics using a β-1,4-endoglucanase gene for Pratylenchus spp. occurring in the Pacific Northwest of North AmericaNematology, 18
(2000)
Molecular 462 characterisation of 18 Pratylenchus species using rDNA Restriction Fragment Length 463 Polymorphism
T. Vrain, D. Wakarchuk, A. Levesque, R. Hamilton (1992)
Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group.Fundamental and applied nematology, 15
R. Floyd, Eyualem Abebe, A. Papert, M. Blaxter (2002)
Molecular barcodes for soil nematode identificationMolecular Ecology, 11
(2020)
414 Root - lesion nematodes of potato : Current status of diagnostics , pathogenicity and 415 management
L. Al-Banna, A. Ploeg, V. Williamson, I. Kaloshian (2004)
Discrimination of six pratylenchus species using PCR and species-specific primers.Journal of nematology, 36 2
Meloidogyne juveniles for identification of pure and mixed samples based on the 433 polymerase chain reaction
J. Flegg, D. Hooper (1970)
Extraction of free-living stages from soil.
G. Yan, R. Smiley, P. Okubara, A. Skantar, S. Easley, J. Sheedy, A. Thompson (2008)
Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil.Plant disease, 92 11
The pitfalls of 385 molecular species identification : a case study within the genus Pratylenchus ( Nematoda : 386 Pratylenchidae )
T. Janssen, G. Karssen, M. Verhaeven, D. Coyne, W. Bert (2016)
Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolutionScientific Reports, 6
L. Waeyenberge, N. Viaene, M. Moens (2009)
Species-specific duplex PCR for the detection of Pratylenchus penetransNematology, 11
(2000)
Molecular 462 characterisation of 18 Pratylenchus species using rDNA Restriction Fragment Length 463
M. Holterman, A. Wurff, S. Elsen, H. Megen, T. Bongers, O. Holovachov, J. Bakker, J. Helder (2006)
Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown Clades.Molecular biology and evolution, 23 9
G. Yan, R. Smiley, P. Okubara, A. Skantar, C. Reardon (2013)
Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil.Plant disease, 97 6
C. Oliveira, V. Blok, R. Neilson, Tomasz Mroz, D. Roberts (2017)
Hydrolysis probe-based PCR for detection of Pratylenchus crenatus, P. neglectus and P. penetransNematology, 19
A. Coomans (2000)
Nematode systematics: past, present and future.Nematology, 2
Benjamin Williams, Bertold Schrank, Chau Huynh, Ratna Shownkeent, Robert Waterston (1992)
A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.Genetics, 131 3
P. Speijer, D. Waele, R. Fogain, S. Gowen, J. Pinochet, N. Price, J. Sarah (1997)
Screening of Musa germplasm for resistance and tolerance to nematodes
V. Orlando, I. Grove, S. Edwards, T. Prior, D. Roberts, R. Neilson, M. Back (2020)
Root‐lesion nematodes of potato: Current status of diagnostics, pathogenicity and managementPlant Pathology, 69
M. Adam, M. Phillips, V. Blok (2007)
Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root‐knot nematode (Meloidogyne spp.)Plant Pathology, 56
SummaryRobust and accurate identification of root-lesion nematodes (Pratylenchus spp.) is an essential step for determining their potential threat to crop yields and, consequently, development of an efficient agronomic management strategy. It is recognised that DNA-based techniques provide rapid identification of a range of plant-parasitic nematodes including Pratylenchus spp. Efficient and repeatable DNA extraction is central to molecular methodologies. Here, six common DNA extraction protocols were compared to evaluate their efficiency to obtain quality DNA samples for Pratylenchus penetrans. Samples with five and ten individuals of P. penetrans were successfully extracted and amplified by all extraction methods tested, whereas samples with a single nematode presented challenges for DNA amplification. Among all methods tested, the DNA extraction protocol with glass beads proved to be efficient for P. penetrans and all other species tested (P. crenatus, P. neglectus and P. thornei), generating high quality DNA at comparatively low cost and with a rapid sample throughput.
Nematology – Brill
Published: Oct 2, 2020
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