Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus

Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus... We have previously identified two secreted glutathione S-transferases (GST) expressed in the pharyngeal gland cell of Bursaphelenchus xylophilus , which are upregulated post infection of the host. This study examines the functional role of GSTs in B. xylophilus biology. We analysed the expression profiles of all predicted GSTs in the genome and the results showed that they belong to kappa and cytosolic subfamilies and the majority are upregulated post infection of the host. A small percentage is potentially secreted and none is downregulated post infection of the host. One secreted protein was confirmed as a functional GST and is within a cluster that showed the highest expression fold change in infection. This enzyme has a protective activity that may involve host defences, namely in the presence of terpenoid compounds and peroxide products. These results suggest that GSTs secreted into the host participate in the detoxification of host-derived defence compounds and enable successful parasitism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus

Nematology , Volume 18 (6): 697 – Jan 1, 2016

Loading next page...
 
/lp/brill/characterization-of-glutathione-s-transferases-from-the-pine-wood-Phdhi7FyQP

References (33)

Publisher
Brill
Copyright
Copyright 2016 by Koninklijke Brill NV, Leiden, The Netherlands.
Subject
Articles
ISSN
1388-5545
eISSN
1568-5411
DOI
10.1163/15685411-00002985
Publisher site
See Article on Publisher Site

Abstract

We have previously identified two secreted glutathione S-transferases (GST) expressed in the pharyngeal gland cell of Bursaphelenchus xylophilus , which are upregulated post infection of the host. This study examines the functional role of GSTs in B. xylophilus biology. We analysed the expression profiles of all predicted GSTs in the genome and the results showed that they belong to kappa and cytosolic subfamilies and the majority are upregulated post infection of the host. A small percentage is potentially secreted and none is downregulated post infection of the host. One secreted protein was confirmed as a functional GST and is within a cluster that showed the highest expression fold change in infection. This enzyme has a protective activity that may involve host defences, namely in the presence of terpenoid compounds and peroxide products. These results suggest that GSTs secreted into the host participate in the detoxification of host-derived defence compounds and enable successful parasitism.

Journal

NematologyBrill

Published: Jan 1, 2016

Keywords: detoxification metabolism; effectors; plant-parasitic nematode; terpenoid compounds

There are no references for this article.