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Enzyme phenotypes and genetic diversity of root-knot nematodes parasitising Musa in Brazil

Enzyme phenotypes and genetic diversity of root-knot nematodes parasitising Musa in Brazil AbstractEsterase phenotypes were used to characterise 25 populations of Meloidogynespp. collected on bananas from different banana producing areas in Brazil,using a simplified technique for routine analysis. Meloidogyne javanica, M.incognita, M. arenaria, and Meloidogyne spp. were detected at percentages of61.7, 32.2, 4.3 and 1.8, respectively. The Meloidogyne populationsrepresented mixed species in 80% of the samples, M. javanica and M.incognita prevailing under field conditions. Genetic analyses were conductedusing RAPD markers which provided, alone or in combination, reliablepolymorphisms both between and within species. Based on the presence orabsence of bands, RAPD analysis of the data resulted in clustering ofspecies and isolates congruent with esterase phenotype characterisation. Theintraspecific variability in M. javanica, M. incognita and M. arenariarepresented 29.1, 19.5 and 40.2% of the polymorphic amplified fragments,respectively. A general lack of correlation was observed between bananacultivar group, geographical origin and differentiation of thenematodes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Enzyme phenotypes and genetic diversity of root-knot nematodes parasitising Musa in Brazil

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References (24)

Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1388-5545
eISSN
1568-5411
DOI
10.1163/156854104323072964
Publisher site
See Article on Publisher Site

Abstract

AbstractEsterase phenotypes were used to characterise 25 populations of Meloidogynespp. collected on bananas from different banana producing areas in Brazil,using a simplified technique for routine analysis. Meloidogyne javanica, M.incognita, M. arenaria, and Meloidogyne spp. were detected at percentages of61.7, 32.2, 4.3 and 1.8, respectively. The Meloidogyne populationsrepresented mixed species in 80% of the samples, M. javanica and M.incognita prevailing under field conditions. Genetic analyses were conductedusing RAPD markers which provided, alone or in combination, reliablepolymorphisms both between and within species. Based on the presence orabsence of bands, RAPD analysis of the data resulted in clustering ofspecies and isolates congruent with esterase phenotype characterisation. Theintraspecific variability in M. javanica, M. incognita and M. arenariarepresented 29.1, 19.5 and 40.2% of the polymorphic amplified fragments,respectively. A general lack of correlation was observed between bananacultivar group, geographical origin and differentiation of thenematodes.

Journal

NematologyBrill

Published: Jan 1, 2004

Keywords: RAPD.; MELOIDOGYNE SPP; BIOCHEMICAL IDENTIFICATION; ELECTROPHORESIS

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