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Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida

Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera... AbstractA full length cDNA clone was isolated by screening a mixed stage Globoderapallida expression library using the monospecific polyclonal antibodyIACR-PC320. Sequence comparison indicated that the predicted 332 amino acidprotein encoded by the cDNA was a member of the annexin gene family namedgp-nex and very similar to annexins found in other nematode species.Annexins are calcium-dependent phospholipid binding proteins characterisedby four repeated domains approximately 70 amino acids in length. Thepredicted amino acid sequence of the protein did not contain an N-terminalsecretion signal peptide; however, the protein was shown to be present inexcretory/secretory products from G. pallida second stage juveniles treatedwith the neurotransmitter 5 methoxy-N, N dimethyl tryptamine. Gp-nex encodesa protein of 35 kDa and was immunolocalised in the amphids, genitalprimordium and in the constraining muscles above and below the metacorpuspump chamber of G. pallida second stage juveniles. It is also present ineggs and adult females of the two species of the potato cyst nematodes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1388-5545
eISSN
1568-5411
DOI
10.1163/156854101300106883
Publisher site
See Article on Publisher Site

Abstract

AbstractA full length cDNA clone was isolated by screening a mixed stage Globoderapallida expression library using the monospecific polyclonal antibodyIACR-PC320. Sequence comparison indicated that the predicted 332 amino acidprotein encoded by the cDNA was a member of the annexin gene family namedgp-nex and very similar to annexins found in other nematode species.Annexins are calcium-dependent phospholipid binding proteins characterisedby four repeated domains approximately 70 amino acids in length. Thepredicted amino acid sequence of the protein did not contain an N-terminalsecretion signal peptide; however, the protein was shown to be present inexcretory/secretory products from G. pallida second stage juveniles treatedwith the neurotransmitter 5 methoxy-N, N dimethyl tryptamine. Gp-nex encodesa protein of 35 kDa and was immunolocalised in the amphids, genitalprimordium and in the constraining muscles above and below the metacorpuspump chamber of G. pallida second stage juveniles. It is also present ineggs and adult females of the two species of the potato cyst nematodes.

Journal

Nematology – Brill

Published: Jan 1, 2001

Keywords: AMPHIDS; GENITAL PRIMORDIUM; CAENORHABDITIS ELEGANS; SECRETION

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