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- Publisher
- Brill
- Copyright
- Copyright © Koninklijke Brill NV, Leiden, The Netherlands
- ISSN
- 0011-216X
- eISSN
- 1568-5403
- DOI
- 10.1163/156854000504165
- Publisher site
- See Article on Publisher Site
Abstract
IMPROVED ISOLATION OF THE CRUSTACEAN HYPERGLYCEMIC HORMONE-CONTAINING GRANULES FROM SINUS GLANDS OF CARCINUS MAENAS (LINNAEUS, 1758) BY PETER P. JAROS 1,4 ), ALBERTO HUBERMAN 2 ), DIETRICH SEDLMEIER 3 ) and RAINER KELLER 3 ) 1 ) Abteilung Zoophysiologie, Carl von Ossietzky Universität Oldenburg, Postfach 2503, D-26111 Oldenburg, Germany 2 ) Instituto Nacional de la Nutrición “S. Zubirán”, Dept. of Biochemistry, Vasco de Quiroga 15, Tlalpan, Mexico D.F. 14000, Mexico 3 ) Institut für Zoophysiologie, Rheinische Friedrich Wilhelm Universität Bonn, Endenicher Allee 11-13, D-53115 Bonn, Germany ABSTRACT An improved neurosecretory granule (NSG) preparation of the shore crab, Carcinus maenas sinus glands, enriched for members of the crustacean hyperglycemic hormone family (CHH) and almost devoid of contaminating subcellular fractions, was obtained by differential and density- gradient centrifugations. CHH was determined in an aliquot of each fraction by means of a speciŽ c radioimmunoassay, and purity of the fractions by means of electron microscopy of pellet sections from duplicate tubes. Fraction F 3 from the Ž rst discontinuous gradient centrifugation was enriched for CHH-containing NSG and puriŽ ed on a second, shallower discontinuous gradient, and fraction F 3b proved to contain solely CHH granules as revealed by EM and RIA criteria.
Journal
Crustaceana – Brill
Published: Jan 1, 2000