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/lp/brill/development-and-characterization-of-twelve-new-polymorphic-6KlIXa5dHT
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- Publisher
- Brill
- Copyright
- © Koninklijke Brill NV, Leiden, The Netherlands
- Subject
- Short Notes
- ISSN
- 0173-5373
- eISSN
- 1568-5381
- DOI
- 10.1163/15685381-00002926
- Publisher site
- See Article on Publisher Site
Abstract
Twelve novel polymorphic microsatellite loci were isolated and characterized for the Iberian ribbed newt, Pleurodeles waltl (Caudata, Salamandridae). The distribution of this newt ranges from central and southern Iberia to northwestern Morocco. Polymorphism of these novel loci was tested in 40 individuals from two Iberian populations and compared with previously published markers. The number of alleles per locus ranged from two to eight. Observed and expected heterozygosity ranged from 0.13 to 0.57 and from 0.21 to 0.64, respectively. Cross-species amplification was tested in Pleurodeles nebulosus , which is listed as Vulnerable by the IUCN. Eight new and seven previously published loci amplified successfully in that species and thus represent a valuable conservation tool. The novel microsatellites will be useful for a better understanding of the population dynamics, demography, genetic structure, and evolutionary history of Pleurodeles waltl and P. nebulosus .
Journal
Amphibia-Reptilia – Brill
Published: Jan 1, 2014
Keywords: amphibians; Iberian Peninsula; microsatellites; North Africa; Pleurodeles waltl ; Pleurodeles nebulosus ; polymorphism