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Recognition of Low-Temperature Infective Isolates of the Entomopathogenic Nematode Heterorhabditis Spp. (Rhabditida: Heterorhabditidae)

Recognition of Low-Temperature Infective Isolates of the Entomopathogenic Nematode... RECOGNITION OF LOW-TEMPERATURE INFECTIVE ISOLATES OF THE ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS SPP. (RHABDITIDA: HETERORHABDITIDAE) BY C. T. GRIFFIN and M. J. DOWNES Department of Biology, St. Patrick's College, Maynooth, Co. Kildare, Ireland A laboratory test for primary screening of large numbers of Heterorhabditis isolates for low- temperature infectivity is described. The cold active isolate HF85 was used for reference. Prelimi- nary studies showed that, at 9°C, Galleria mellonella in sand columns or Tenebrio molitor on filter paper identified much the same group of isolates as cold-infective. Seven of the ten isolates identified as cold-infective on the basis of the number of nematodes established in Galleria were also identified on the basis of percentage Galleria killed. Performance of Heterorhabditis isolates in the Galleria bioassay was compared at 9, 15 and 20°C. There was a significant positive correlation between infectivity at 9°C and infectivity at 15°C but not between 9°C and 20°C. Of 151 Heterorhabditis isolates screened at 9°C, 29 were sufficiently infective to justify selection for further testing. The infectivity of juveniles of various isolates in bioassay at 9°C was compared with their motility (rate of movement and proportion of nematodes active) at that temperature. There was a significant positive http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Recognition of Low-Temperature Infective Isolates of the Entomopathogenic Nematode Heterorhabditis Spp. (Rhabditida: Heterorhabditidae)

Griffin, C.T.; Downes, M.J.
Nematologica , Volume 40 (1-4): 10 – Jan 1, 1994
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/003525994X00076
Publisher site
See Article on Publisher Site

Abstract

RECOGNITION OF LOW-TEMPERATURE INFECTIVE ISOLATES OF THE ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS SPP. (RHABDITIDA: HETERORHABDITIDAE) BY C. T. GRIFFIN and M. J. DOWNES Department of Biology, St. Patrick's College, Maynooth, Co. Kildare, Ireland A laboratory test for primary screening of large numbers of Heterorhabditis isolates for low- temperature infectivity is described. The cold active isolate HF85 was used for reference. Prelimi- nary studies showed that, at 9°C, Galleria mellonella in sand columns or Tenebrio molitor on filter paper identified much the same group of isolates as cold-infective. Seven of the ten isolates identified as cold-infective on the basis of the number of nematodes established in Galleria were also identified on the basis of percentage Galleria killed. Performance of Heterorhabditis isolates in the Galleria bioassay was compared at 9, 15 and 20°C. There was a significant positive correlation between infectivity at 9°C and infectivity at 15°C but not between 9°C and 20°C. Of 151 Heterorhabditis isolates screened at 9°C, 29 were sufficiently infective to justify selection for further testing. The infectivity of juveniles of various isolates in bioassay at 9°C was compared with their motility (rate of movement and proportion of nematodes active) at that temperature. There was a significant positive

Journal

Nematologica – Brill

Published: Jan 1, 1994

Keywords: screen; entomopathogenic nematode; infectivity; bioassay; motility; cold active

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