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Optimizing the Use of a Voided Urine Cytology Specimen as Control Material for Anti-BK Virus Immunohistochemical Staining

Optimizing the Use of a Voided Urine Cytology Specimen as Control Material for Anti-BK Virus... Dear Editor,One of the obstacles faced in renal pathology is the limited availability of core needle biopsy material or commercial supplies that can be used as control tissue for anti-BK virus immunohistochemistry (IHC). BK virus can be especially detrimental to immunosuppressed renal transplant recipients, leading to premature allograft failure. Identifying virally infected epithelial cells on hematoxylin and eosin staining can sometimes be difficult [1], which is why we often rely on anti-BK virus IHC to support the diagnosis. IHC is the gold standard for diagnosing polyomavirus BK nephropathy [2]. To overcome the obstacle of limited control tissue, we have been using blocks prepared from a voided urine cytology specimen; this specimen was taken from a patient with PCR- documented BK viremia (Fig. 1).Fig. 1.BK-infected urothelial cell on cytology showing an enlarged smudgy nucleus (decoy cell). Papanicolaou stain. ×100 (oil immersion).The specimen used in this process was given to the university renal biopsy service by a local hospital. It was a de-identified refrigerated 1.0-L single-shift voided urine specimen. The study was exempted from review by the local institutional review board.Upon receiving the urine specimen from the hospital, it was immediately processed into blocks by our laboratory, which obviated the need for http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Optimizing the Use of a Voided Urine Cytology Specimen as Control Material for Anti-BK Virus Immunohistochemical Staining

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Publisher
Karger
Copyright
© 2018 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000487792
Publisher site
See Article on Publisher Site

Abstract

Dear Editor,One of the obstacles faced in renal pathology is the limited availability of core needle biopsy material or commercial supplies that can be used as control tissue for anti-BK virus immunohistochemistry (IHC). BK virus can be especially detrimental to immunosuppressed renal transplant recipients, leading to premature allograft failure. Identifying virally infected epithelial cells on hematoxylin and eosin staining can sometimes be difficult [1], which is why we often rely on anti-BK virus IHC to support the diagnosis. IHC is the gold standard for diagnosing polyomavirus BK nephropathy [2]. To overcome the obstacle of limited control tissue, we have been using blocks prepared from a voided urine cytology specimen; this specimen was taken from a patient with PCR- documented BK viremia (Fig. 1).Fig. 1.BK-infected urothelial cell on cytology showing an enlarged smudgy nucleus (decoy cell). Papanicolaou stain. ×100 (oil immersion).The specimen used in this process was given to the university renal biopsy service by a local hospital. It was a de-identified refrigerated 1.0-L single-shift voided urine specimen. The study was exempted from review by the local institutional review board.Upon receiving the urine specimen from the hospital, it was immediately processed into blocks by our laboratory, which obviated the need for

Journal

Acta Cytologica – Karger

Published: Jun 1, 2018

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