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Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers Dear Editor,We would like to thank Dr. Nersesyan for his interest in our work [1]. First, the micronucleus assay in buccal mucosa cells is a very subjective technique able to detect nuclear abnormalities in the oral keratinocytes. Second, some variations when identifying these are always expected. Taken as a whole, the most important finding is the biological significance of the results and not the specific values found. This is because to recognize metanuclear alterations requires extensive scientific training and specific staining. Our research group has struggled to validate the micronucleus assay in oral mucosa cells with reproducible results. For this purpose, the use of specific DNA staining (Feulgen-Fast Green), the number of counted cells (2,000), and the way to dispose the cells on the slide (dropping) are systematically applied in our laboratory as described by Tolbert et al. [2]. The counting criteria of micronucleus and nuclear changes were followed as recommended by Beliën et al. [3]. The statistical method used to compare the differences between the exposed and the control groups was made according to Pereira et al. [4].It is important to stress that this study was part of a large study investigating the smoking, education degree, social levels, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

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Publisher
Karger
Copyright
© 2016 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000452157
Publisher site
See Article on Publisher Site

Abstract

Dear Editor,We would like to thank Dr. Nersesyan for his interest in our work [1]. First, the micronucleus assay in buccal mucosa cells is a very subjective technique able to detect nuclear abnormalities in the oral keratinocytes. Second, some variations when identifying these are always expected. Taken as a whole, the most important finding is the biological significance of the results and not the specific values found. This is because to recognize metanuclear alterations requires extensive scientific training and specific staining. Our research group has struggled to validate the micronucleus assay in oral mucosa cells with reproducible results. For this purpose, the use of specific DNA staining (Feulgen-Fast Green), the number of counted cells (2,000), and the way to dispose the cells on the slide (dropping) are systematically applied in our laboratory as described by Tolbert et al. [2]. The counting criteria of micronucleus and nuclear changes were followed as recommended by Beliën et al. [3]. The statistical method used to compare the differences between the exposed and the control groups was made according to Pereira et al. [4].It is important to stress that this study was part of a large study investigating the smoking, education degree, social levels,

Journal

Acta CytologicaKarger

Published: Jan 1, 2016

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