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Acta Cytologica Three-Dimensional Cytomorphology in Fine Needle Aspiration Biopsy of Medullary Thyroid Carcinoma Tien-Chun Chang, M.D., Ph.D., M.I.A.C., Shu-Mei Lai, M.Sc., Chen-Yuan Wen, Ph.D., Yung-Lien Hsiao and Shih-Horng Huang, M.D. OBJECTIVE: To elucidate three-dimensional (3-D) cy- ings under SEM were correlated with those under LM tomorphology in fine needle aspiration biopsy (FNAB) of and TEM. medullary thyroid carcinoma RESULTS: Under SEM, 3- (MTC). D cytomorphology of MTC STUDY DESIGN: FNAB displayed a disorganized cel- Because MTC has neurosecretory was performed on tumors lular arrangement with in- granules in the cytoplasm and can from five patients with MTC. distinct cell borders in three The aspirate was stained and secrete these granules to the cell cases. The cell surface was observed under a light micro- uneven and had granular surface,...this special 3-D scope (LM). The aspirate was protrusions that correspond- cytomorphology might be helpful also fixed, dehydrated, criti- ed to secretory granules ob- cal point dried, spattered served under TEM. In one in the differential diagnosis. with gold ions and observed case with multiple endocrine with a scanning electron mi- neoplasia type IIB, there croscope (SEM). For transmission electron microscopy were abundant granules on the cell surface. In one case of
Acta Cytologica – Karger
Published: Jan 1, 2011
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