Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Reyes (2010)
Diff-Quik Cytologic Recognition of Chlamydophila psittaci in Orolabial Lesions of Stevens-Johnson SyndromeActa Cytologica, 54
J. Wallace (2002)
Pulmonary blastomycosis: a great masquerader.Chest, 121 3
M. Mendoza, P. Martín-Rabadán, I. Carrión, J. Morillas, G. López-Alonso, M. Díaz‐Rubio (1993)
Helicobacter pylori infection. Rapid diagnosis with brush cytology.Acta cytologica, 37 2
M. Stanley, C. Horwitz, L. Burton, J. Weisser (1990)
Negative images of bacilli and mycobacterial infection: A study of fine‐needle aspiration smears from lymph nodes in patients with aidsDiagnostic Cytopathology, 6
D. Larone (1976)
Medically Important Fungi: A Guide to Identification
S. Hirschowitz, D. Mandell, R. Nieberg, K. Carson (1994)
The alcohol-fixed Diff-Quik stain. A novel rapid stain for the immediate interpretation of fine needle aspiration specimens.Acta cytologica, 38 4
Glen Axelson, T. Giorgadze, G. Youngberg (2007)
Evaluation of the use of Congo red staining in the differential diagnosis of Candida vs. various other yeast‐form fungal organismsJournal of Cutaneous Pathology, 35
Rezhan Hussein, Saad Khan, F. Levy, J. Mehta, F. Sarubbi (2009)
Blastomycosis in the mountainous region of northeast Tennessee.Chest, 135 4
A. Domfeh, L. Nodit, J. Gradowski, S. Bastacky (2007)
Mycobacterium kansasii Infection Diagnosed by Pleural Fluid CytologyActa Cytologica, 51
Grace Ang, W. Janda, R. Novak, L. Gerardo (1993)
Negative images of mycobacteria in aspiration biopsy smears from the lymph node of a patient with acquired immunodeficiency syndrome (AIDS): Report of a case and a review of the literatureDiagnostic Cytopathology, 9
T. Carmona, E. Muñoz, M. Abad, J. Paz, F. Gómez, M. Alonso, A. Sánchez, A. Bullón (1995)
Usefulness of antral brushing samples stained with Diff-Quik in the cytologic diagnosis of Helicobacter pylori. A comparative methodologic study.Acta cytologica, 39 4
L. Casanova-Cardiel, R. Cedillo-Rivera, G. Garduño-Rodríguez, O. Muñoz (1996)
[Comparison of 2 dyes in the detection of Pneumocystis carinii].Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 48 6
A. Kyriazis, A. Kyriazis (1993)
Incidence and distribution of opportunistic lung infections in aids patients related to intravenous drug use: A study of bronchoalveolar lavage cytology by the diff‐quik stainDiagnostic Cytopathology, 9
Background: Blastomycosis is caused by a dimorphic fungus that can be difficult to diagnose in certain situations. The disease is sometimes serious and can be deadly. Diagnosis by fungal serology and urinary antigens is not easy to establish and unreliable. Culture is also time-consuming and is not easy to perform. Thus, documentation of such an organism on cytology offers a quick and cost-effective alternative. This report describes for the first time identification of the ‘negative image’ of Blastomyces budding yeast. Case: A 79-year-old man presented with a left lung nodule associated with mediastinal and hilar lymphadenopathy. Fine needle aspiration was performed, and a ‘negative image’ of a yeast with wide base budding was noted on Diff-Quik (DQ)-stained smears. Blastomyces species were confirmed with periodic acid-Schiff fungal stain. Additionally, the fungal capsule contained focally polarizable material on Congo red stain and lacked mucin with mucicarmine stain. Conclusion:Blastomyces yeast forms can be easily identified with DQ staining by their ‘negative image’. This feature can be utilized as a quick and cost-effective cytological characteristic to further triage these specimens for confirmation. The information can be of great value to clinicians in making appropriate clinical decisions.
Acta Cytologica – Karger
Published: Jan 1, 2011
Keywords: Blastomycosis; Negative image; Diff-Quik stain
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.