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Papanicolaou Stain

Papanicolaou Stain TECHNIQUES Is It Economical to Switch to Rapid, Economical, Acetic Acid, Swati B. Dighe, M.Sc., C.T., Dulhan Ajit, M.Sc., C.T., Saleem Pathuthara, B.Sc., D.M.L.T., C.T., and Roshni Chinoy, M.D., D.C.P. Objective Conclusion To standardize an inexpensive and rapid Papanicolaou REAP is a rapid, cost-effective alternative to Papanicolaou staining technique with limited ethanol usage. stain. Though low stain penetration in large cell clusters is a limitation, final interpretation was not compromised. Study Design (Acta Cytol 2006;50:643–646) Smears from 200 patients were collected (2 per patient) and fixed in methanol. Half were Keywords: Papanicolaou subjected to conventional Pa- smear, mass screening, panicolaou and half to stain- stains, laboratory diagnosis, REAP stain provides excellent ing with rapid, economical, REAP stain. screening and rapid diagnosis acetic acid Papanicolaou with limited cost. (REAP) stain. In REAP, pre- apanicolaou staining is OG6 and post-OG6 and post- Pthe best staining tech- EA36 ethanol baths were re- nique for cytologic smears, placed by 1% acetic acid and Scott’s tap water with tap as it yields a polychromatic, transparent staining reac- water. Hematoxylin was preheated to 60°C. Final dehy- tion with crisp nuclear and cytoplasmic features. dration was with methanol. REAP smears were compared However, it utilizes a http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Papanicolaou Stain

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References (4)

Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000326034
Publisher site
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Abstract

TECHNIQUES Is It Economical to Switch to Rapid, Economical, Acetic Acid, Swati B. Dighe, M.Sc., C.T., Dulhan Ajit, M.Sc., C.T., Saleem Pathuthara, B.Sc., D.M.L.T., C.T., and Roshni Chinoy, M.D., D.C.P. Objective Conclusion To standardize an inexpensive and rapid Papanicolaou REAP is a rapid, cost-effective alternative to Papanicolaou staining technique with limited ethanol usage. stain. Though low stain penetration in large cell clusters is a limitation, final interpretation was not compromised. Study Design (Acta Cytol 2006;50:643–646) Smears from 200 patients were collected (2 per patient) and fixed in methanol. Half were Keywords: Papanicolaou subjected to conventional Pa- smear, mass screening, panicolaou and half to stain- stains, laboratory diagnosis, REAP stain provides excellent ing with rapid, economical, REAP stain. screening and rapid diagnosis acetic acid Papanicolaou with limited cost. (REAP) stain. In REAP, pre- apanicolaou staining is OG6 and post-OG6 and post- Pthe best staining tech- EA36 ethanol baths were re- nique for cytologic smears, placed by 1% acetic acid and Scott’s tap water with tap as it yields a polychromatic, transparent staining reac- water. Hematoxylin was preheated to 60°C. Final dehy- tion with crisp nuclear and cytoplasmic features. dration was with methanol. REAP smears were compared However, it utilizes a

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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