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Leishman-Giemsa Cocktail

Leishman-Giemsa Cocktail Other Techniques An Effective Romanowsky Stain for Air-Dried Cytologic Smears Rajendra S. Garbyal, M.D., Neeta Agarwal, M.D., and Prachi Kumar, M.D. Objective Keywords: stains, May-Grünwald-Giemsa stain, To develop an economical, quick, readily available and sim- Diff-Quik, LG cocktail. ple alternative to May-Grünwald-Giemsa (MGG) stain and so to explore the combination of Leishman and Giemsa omanowsky stains are routinely used for air-dried stain (LG cocktail). Rcytology smears. They stain the cell cytoplasm and identify ground substances by metachromasia. Study Design Nuclear chromatin pattern One wet-fixed and 2 air-dried can also be demonstrated smears were prepared from with a correctly prepared We recommend use of this cocktail 720 cases during the period stain. Many combinations as it can reduce the cost and time January 2003–November of Romanowsky stains, 2004. The LG cocktail and of cytologic practice without such as May-Grünwald- MGG stain were used on the Giemsa (MGG), Wright- compromising the quality air-dried smears and Papani- Giemsa and a commercially of staining. colaou stain in wet-fixed available stain kit (a modi- smears. Diagnoses of the cases fied Wright stain, Diff- were made using the LG cock- Quik) have also been used. tail–stained smears, and then its diagnostic efficacy was Leishman’s stain http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Leishman-Giemsa Cocktail

Garbyal, Rajendra S.; Agarwal, Neeta; Kumar, Prachi
Acta Cytologica , Volume 50 (4): 4 – Jan 1, 2011
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References (8)

Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000325981
Publisher site
See Article on Publisher Site

Abstract

Other Techniques An Effective Romanowsky Stain for Air-Dried Cytologic Smears Rajendra S. Garbyal, M.D., Neeta Agarwal, M.D., and Prachi Kumar, M.D. Objective Keywords: stains, May-Grünwald-Giemsa stain, To develop an economical, quick, readily available and sim- Diff-Quik, LG cocktail. ple alternative to May-Grünwald-Giemsa (MGG) stain and so to explore the combination of Leishman and Giemsa omanowsky stains are routinely used for air-dried stain (LG cocktail). Rcytology smears. They stain the cell cytoplasm and identify ground substances by metachromasia. Study Design Nuclear chromatin pattern One wet-fixed and 2 air-dried can also be demonstrated smears were prepared from with a correctly prepared We recommend use of this cocktail 720 cases during the period stain. Many combinations as it can reduce the cost and time January 2003–November of Romanowsky stains, 2004. The LG cocktail and of cytologic practice without such as May-Grünwald- MGG stain were used on the Giemsa (MGG), Wright- compromising the quality air-dried smears and Papani- Giemsa and a commercially of staining. colaou stain in wet-fixed available stain kit (a modi- smears. Diagnoses of the cases fied Wright stain, Diff- were made using the LG cock- Quik) have also been used. tail–stained smears, and then its diagnostic efficacy was Leishman’s stain

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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