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MOLECULAR TECHNIQUES Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears Morelva Toro de Méndez, Ph.D., and Antonio Llombart Bosch, M.D., Ph.D. Objective lowed by HPV18 (20%), HPV6 (10%) and HPV58 To evaluate the effectiveness of the short PCR fragment (10%). We also found HR-HPV45, 52, 58 and 68 and 10–line probe assay (SPF10-LiPA) system in detection of LR-HPV6, 11 and 70 viral DNA types. These multiple in- human papillomavirus (HPV) in abnormal Papanicolaou- fections were detected with greater frequencies in atypical stained cervicovaginal smears. squamous cells of undeter- mined significance and in the Study Design low-grade squamous intraep- Our results confirm that it is We included 20 abnormal ithelial lesions. possible to identify the HPV DNA Papanicolaou-stained cervico- Conclusion sequences from abnormal vaginal smears. Samples were tested for HPV DNA detec- The SPF10-LiPA system is Papanicolaou-stained cervical tion and genotyping using the efficient, sensitive, fast and smears using the SPF10–LiPA SPF10-LiPA system. simple for detecting and geno- typing HPV DNA in abnor- system. . . . Results mal Papanicolaou-stained cer- All samples amplified the vicovaginal smears, which INF150 gene control. In 2 of 20 cases,
Acta Cytologica – Karger
Published: Jan 1, 2011
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