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Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears

Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR... MOLECULAR TECHNIQUES Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears Morelva Toro de Méndez, Ph.D., and Antonio Llombart Bosch, M.D., Ph.D. Objective lowed by HPV18 (20%), HPV6 (10%) and HPV58 To evaluate the effectiveness of the short PCR fragment (10%). We also found HR-HPV45, 52, 58 and 68 and 10–line probe assay (SPF10-LiPA) system in detection of LR-HPV6, 11 and 70 viral DNA types. These multiple in- human papillomavirus (HPV) in abnormal Papanicolaou- fections were detected with greater frequencies in atypical stained cervicovaginal smears. squamous cells of undeter- mined significance and in the Study Design low-grade squamous intraep- Our results confirm that it is We included 20 abnormal ithelial lesions. possible to identify the HPV DNA Papanicolaou-stained cervico- Conclusion sequences from abnormal vaginal smears. Samples were tested for HPV DNA detec- The SPF10-LiPA system is Papanicolaou-stained cervical tion and genotyping using the efficient, sensitive, fast and smears using the SPF10–LiPA SPF10-LiPA system. simple for detecting and geno- typing HPV DNA in abnor- system. . . . Results mal Papanicolaou-stained cer- All samples amplified the vicovaginal smears, which INF150 gene control. In 2 of 20 cases, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears

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Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000325382
Publisher site
See Article on Publisher Site

Abstract

MOLECULAR TECHNIQUES Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears Morelva Toro de Méndez, Ph.D., and Antonio Llombart Bosch, M.D., Ph.D. Objective lowed by HPV18 (20%), HPV6 (10%) and HPV58 To evaluate the effectiveness of the short PCR fragment (10%). We also found HR-HPV45, 52, 58 and 68 and 10–line probe assay (SPF10-LiPA) system in detection of LR-HPV6, 11 and 70 viral DNA types. These multiple in- human papillomavirus (HPV) in abnormal Papanicolaou- fections were detected with greater frequencies in atypical stained cervicovaginal smears. squamous cells of undeter- mined significance and in the Study Design low-grade squamous intraep- Our results confirm that it is We included 20 abnormal ithelial lesions. possible to identify the HPV DNA Papanicolaou-stained cervico- Conclusion sequences from abnormal vaginal smears. Samples were tested for HPV DNA detec- The SPF10-LiPA system is Papanicolaou-stained cervical tion and genotyping using the efficient, sensitive, fast and smears using the SPF10–LiPA SPF10-LiPA system. simple for detecting and geno- typing HPV DNA in abnor- system. . . . Results mal Papanicolaou-stained cer- All samples amplified the vicovaginal smears, which INF150 gene control. In 2 of 20 cases,

Journal

Acta CytologicaKarger

Published: Jan 1, 2011

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