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Rapid Detection of Matrix Metalloproteinase-7 and Carcinoembryonic Antigen mRNA Expression in Intraoperative Pleural Lavage Samples Using the Reverse Transcriptase Loop-Mediated Isothermal Amplification Method

Rapid Detection of Matrix Metalloproteinase-7 and Carcinoembryonic Antigen mRNA Expression in... MOLECULAR TECHNIQUES Rapid Detection of Matrix Metalloproteinase-7 and Carcinoembryonic Antigen mRNA Expression in Intraoperative Pleural Lavage Samples Using the Reverse Transcriptase Loop-Mediated Isothermal Amplification Method Satoshi Ikeda, Ph.D., Masaharu Inagaki, M.D., Ph.D., Kazuhiko Takabe, M.D., Jiro Kumagai, M.D., Ph.D., and Keiko Suzuki, M.D. Objective Results To detect the RNA of the tumor origin in intraoperative MMP-7 mRNA was detected in cancer tissues from 76 pleural lavage accurately by examining using rapid nucleic (96%) of those cases, while it was also detected in the intra- acid amplification method because patients with lung cancer operative pleural lavage samples of 23 (30%) of those 76 often have recurrence detected cases. In addition, CEA by a cytologic examination of mRNA was detected in cancer cancer cells in intraoperative tissues from 55 (70%) cases With the present method, findings pleural lavage. and in 8 (15%) of the lavage were obtained during the operation samples. MMP-7 mRNA– Study Design within 1 hour from sampling. positive cases showed a signifi- We used reverse transcriptase cantly positive correlation with loop-mediated isothermal am- the p and n factors, and mod- plification (RT-LAMP), which enables rapid gene amplifi- erate to poor differentiation. Three cases shown to be posi- cation, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Rapid Detection of Matrix Metalloproteinase-7 and Carcinoembryonic Antigen mRNA Expression in Intraoperative Pleural Lavage Samples Using the Reverse Transcriptase Loop-Mediated Isothermal Amplification Method

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References (21)

Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000325309
Publisher site
See Article on Publisher Site

Abstract

MOLECULAR TECHNIQUES Rapid Detection of Matrix Metalloproteinase-7 and Carcinoembryonic Antigen mRNA Expression in Intraoperative Pleural Lavage Samples Using the Reverse Transcriptase Loop-Mediated Isothermal Amplification Method Satoshi Ikeda, Ph.D., Masaharu Inagaki, M.D., Ph.D., Kazuhiko Takabe, M.D., Jiro Kumagai, M.D., Ph.D., and Keiko Suzuki, M.D. Objective Results To detect the RNA of the tumor origin in intraoperative MMP-7 mRNA was detected in cancer tissues from 76 pleural lavage accurately by examining using rapid nucleic (96%) of those cases, while it was also detected in the intra- acid amplification method because patients with lung cancer operative pleural lavage samples of 23 (30%) of those 76 often have recurrence detected cases. In addition, CEA by a cytologic examination of mRNA was detected in cancer cancer cells in intraoperative tissues from 55 (70%) cases With the present method, findings pleural lavage. and in 8 (15%) of the lavage were obtained during the operation samples. MMP-7 mRNA– Study Design within 1 hour from sampling. positive cases showed a signifi- We used reverse transcriptase cantly positive correlation with loop-mediated isothermal am- the p and n factors, and mod- plification (RT-LAMP), which enables rapid gene amplifi- erate to poor differentiation. Three cases shown to be posi- cation,

Journal

Acta CytologicaKarger

Published: Jan 1, 2011

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