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Rapid Multiple Immunocytochemical Staining Method Using Microwave Irradiation for Intraoperative Cytology

Rapid Multiple Immunocytochemical Staining Method Using Microwave Irradiation for Intraoperative... TECHNIQUES Rapid Multiple Immunocytochemical Staining Method Using Microwave Irradiation for Intraoperative Cytology Atsushi Furuhata, C.T.(IAC), Noriyoshi Sueyoshi, M.T., Hidetake Kurihara, Ph.D., Kazuhiro Sakamoto, M.D., Ph.D., Toshiki Kamano, M.D., Ph.D., and Okio Hino, M.D., Ph.D. Objective Results To develop a more rapid and accurate staining procedure for High rates of positive cells (83–87%) with intense im- intraoperative cytology through an assessment of a rapid munoreactions were obtained using a rapid process with pri- multiple immunocytochemical staining method using mi- mary antibody concentrations that were 2 times higher than crowave irradiation. those used for standard incubation at room temperature (82–86%). The staining under these conditions was com- Study Design pleted within 19 minutes. A preliminary test of a triple Adenocarcinoma cells in the The total processing time for multiple immunostaining method us- peritoneal washings were also ing microwave irradiation intensely stained under these immunocytochemical staining was was performed to determine conditions. dramatically shortened using our optimal incubating conditions Conclusion for primary antibodies against MW-3 method. MOC-31, BerEP4 and carci- Using our microwave method, noembryonic antigen. The test the processing time was dra- samples were adenocarcinoma cells obtained by washings matically shortened, and intense and sensitive immunoreac- from 10 resected colorectal cancer http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Rapid Multiple Immunocytochemical Staining Method Using Microwave Irradiation for Intraoperative Cytology

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References (20)

Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000325036
Publisher site
See Article on Publisher Site

Abstract

TECHNIQUES Rapid Multiple Immunocytochemical Staining Method Using Microwave Irradiation for Intraoperative Cytology Atsushi Furuhata, C.T.(IAC), Noriyoshi Sueyoshi, M.T., Hidetake Kurihara, Ph.D., Kazuhiro Sakamoto, M.D., Ph.D., Toshiki Kamano, M.D., Ph.D., and Okio Hino, M.D., Ph.D. Objective Results To develop a more rapid and accurate staining procedure for High rates of positive cells (83–87%) with intense im- intraoperative cytology through an assessment of a rapid munoreactions were obtained using a rapid process with pri- multiple immunocytochemical staining method using mi- mary antibody concentrations that were 2 times higher than crowave irradiation. those used for standard incubation at room temperature (82–86%). The staining under these conditions was com- Study Design pleted within 19 minutes. A preliminary test of a triple Adenocarcinoma cells in the The total processing time for multiple immunostaining method us- peritoneal washings were also ing microwave irradiation intensely stained under these immunocytochemical staining was was performed to determine conditions. dramatically shortened using our optimal incubating conditions Conclusion for primary antibodies against MW-3 method. MOC-31, BerEP4 and carci- Using our microwave method, noembryonic antigen. The test the processing time was dra- samples were adenocarcinoma cells obtained by washings matically shortened, and intense and sensitive immunoreac- from 10 resected colorectal cancer

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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