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Studies of Lymphocytes and Their Derivative Cells in vitro

Studies of Lymphocytes and Their Derivative Cells in vitro Acta haemal. 37: 42-52 (1967) D epartm ent of Clinical H aem atology, T h e U niversity and R oyal Infirm ary, M anchester Studies of Lymphocytes and Their Derivative Cells in vitro I I . Enzyme Cytochemistry J . G o u g h * a n d M. W. E lves Lymphocytes are capable of transforming into macrophages in vitro (16), and when stimulated with phytohaem agglutinin (PHA) may develop into nucleolated pyroninophilic cells which have a morphological resemblance to haematological stem-cells (7, 12, 22). Certain biochemical constituents have been studied in these various cells (15), and in this study the activity o f various enzymes will be com pared in the lymphocyte, the m acrophage, and the PHA-transformed cell. Methods Smears of leucocytes, m acrophages a n d PHA-transform ed cells were p rep a red as previously described (15). Alkaline phosphatase. A modified technique was used (11), w hich is based on K ap- low’s m ethod (21). Acid phosphatase. T h e s ta n d a rd m ethod of G rogg and Pearse (17) was used w ith smears fixed in form alin v a p o u r for 5 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Haematologica Karger

Studies of Lymphocytes and Their Derivative Cells in vitro

Gough, J.; Elves, M.W.
Acta Haematologica , Volume 37 (1): 11 – Jan 1, 2009
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Publisher
Karger
Copyright
© 1967 S. Karger AG, Basel
ISSN
0001-5792
eISSN
1421-9662
DOI
10.1159/000209051
Publisher site
See Article on Publisher Site

Abstract

Acta haemal. 37: 42-52 (1967) D epartm ent of Clinical H aem atology, T h e U niversity and R oyal Infirm ary, M anchester Studies of Lymphocytes and Their Derivative Cells in vitro I I . Enzyme Cytochemistry J . G o u g h * a n d M. W. E lves Lymphocytes are capable of transforming into macrophages in vitro (16), and when stimulated with phytohaem agglutinin (PHA) may develop into nucleolated pyroninophilic cells which have a morphological resemblance to haematological stem-cells (7, 12, 22). Certain biochemical constituents have been studied in these various cells (15), and in this study the activity o f various enzymes will be com pared in the lymphocyte, the m acrophage, and the PHA-transformed cell. Methods Smears of leucocytes, m acrophages a n d PHA-transform ed cells were p rep a red as previously described (15). Alkaline phosphatase. A modified technique was used (11), w hich is based on K ap- low’s m ethod (21). Acid phosphatase. T h e s ta n d a rd m ethod of G rogg and Pearse (17) was used w ith smears fixed in form alin v a p o u r for 5

Journal

Acta Haematologica – Karger

Published: Jan 1, 2009

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