Immunofluorescent Diagnosis of Acute Lymphoblastic Leukaemia
Immunofluorescent Diagnosis of Acute Lymphoblastic Leukaemia
Marmont, A.M.; Santini, G.; Bacigalupo, A.; Damasio, E.E.; Giordano, D.
1975-01-01 00:00:00
When leukaemic lymphoblasts from acute lymphatic leukaemia were reacted with animal anti-human lymphocyte globulin (AHLG), and subsequently with the appropriate, fluorescein-isothiocyanate-conjugated antibodies, a brilliant membrane fluorescence was observed, which persisted up to 5 log titres, breaking from rings into spots at higher dilutions. The best results were obtained with fresh suspensions and incident illumination. Cross-reactions with mature and immature myeloid cells with the unabsorbed antisera could be abolished by repeated preabsorbtion of AHLG with packed leukocyte preparations from chronic and acute myeloid leukaemias. Thus, it appears that this approach is susceptible of improving the identification of leukaemic lymphoblasts, and may be extended, by means of appropriate antisera, to the acute leukaemias in general.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngActa HaematologicaKargerhttp://www.deepdyve.com/lp/karger/immunofluorescent-diagnosis-of-acute-lymphoblastic-leukaemia-pAMoxXOlK0
Immunofluorescent Diagnosis of Acute Lymphoblastic Leukaemia
When leukaemic lymphoblasts from acute lymphatic leukaemia were reacted with animal anti-human lymphocyte globulin (AHLG), and subsequently with the appropriate, fluorescein-isothiocyanate-conjugated antibodies, a brilliant membrane fluorescence was observed, which persisted up to 5 log titres, breaking from rings into spots at higher dilutions. The best results were obtained with fresh suspensions and incident illumination. Cross-reactions with mature and immature myeloid cells with the unabsorbed antisera could be abolished by repeated preabsorbtion of AHLG with packed leukocyte preparations from chronic and acute myeloid leukaemias. Thus, it appears that this approach is susceptible of improving the identification of leukaemic lymphoblasts, and may be extended, by means of appropriate antisera, to the acute leukaemias in general.
To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.