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/lp/karger/the-effect-of-diamide-azodicarboxylic-acid-bis-dimethylamide-on-fcGF8vfl25
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- Publisher
- Karger
- Copyright
- © 1982 S. Karger AG, Basel
- ISSN
- 0001-5792
- eISSN
- 1421-9662
- DOI
- 10.1159/000206993
- Publisher site
- See Article on Publisher Site
Abstract
Treatment of human blood platelets with the thiol-oxidizing agent, diamide, causes rapid oxidation of glutathione and alterations in aggregation and release reaction. Moreover, cross-linking of proteins was observed. Three high molecular weight bands of 200 – 240 × 10<sup>3</sup> daltons and a band of 66 × 10<sup>3</sup> daltons were involved in this process. After reduction with dithiotreitol the normal pattern was received. In contrast to the erythrocyte, the cross-linking of platelet proteins was not accompanied by a reorientation of phosphatidylethanolamine in the membrane. Also a considerably smaller effect of diamide on platelet protein sulfhydryl group content was measured.
Journal
Acta Haematologica – Karger
Published: Jan 1, 1982
Keywords: Diamide; Human blood platelets; Phosphatidylethanolamine labeling; SDS electrophoresis; Sulfhydryl groups