Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods: High-Performance Liquid Chromatography and DE-52 Microchromatography
Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods:...
Samperi, P.; Testa, R.; Mancuso, M.; Schilirò, G.
1990-01-01 00:00:00
Hb A<sub>2</sub> was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of β -thalassemia trait and 228 were normal controls. Hb A<sub>2</sub> was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngActa HaematologicaKargerhttp://www.deepdyve.com/lp/karger/comparative-approach-to-the-evaluation-of-hemoglobin-a2-by-two-0h6lpOKhRi
Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods: High-Performance Liquid Chromatography and DE-52 Microchromatography
Hb A<sub>2</sub> was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of β -thalassemia trait and 228 were normal controls. Hb A<sub>2</sub> was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.
Journal
Acta Haematologica
– Karger
Published: Jan 1, 1990
Keywords: Hb A 2; High-performance liquid chromatography; Iron deficiency anemia; β-Thalassemia trait
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