Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods: High-Performance Liquid Chromatography and DE-52 Microchromatography

Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods:... Hb A<sub>2</sub> was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of β -thalassemia trait and 228 were normal controls. Hb A<sub>2</sub> was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Haematologica Karger

Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods: High-Performance Liquid Chromatography and DE-52 Microchromatography

Acta Haematologica , Volume 83 (4): 4 – Jan 1, 1990

Loading next page...
 
/lp/karger/comparative-approach-to-the-evaluation-of-hemoglobin-a2-by-two-0h6lpOKhRi

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Karger
Copyright
© 1990 S. Karger AG, Basel
ISSN
0001-5792
eISSN
1421-9662
DOI
10.1159/000205209
Publisher site
See Article on Publisher Site

Abstract

Hb A<sub>2</sub> was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of β -thalassemia trait and 228 were normal controls. Hb A<sub>2</sub> was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.

Journal

Acta HaematologicaKarger

Published: Jan 1, 1990

Keywords: Hb A 2; High-performance liquid chromatography; Iron deficiency anemia; β-Thalassemia trait

There are no references for this article.