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Fragmentation of Red Cells and Ghosts in Urea Solutions, with a Note on Electrophoresis Patterns

Fragmentation of Red Cells and Ghosts in Urea Solutions, with a Note on Electrophoresis Patterns P onder, E. and R . P onder: Acta hacm at. 12, 282 -290, 1934. From T he Nassau Hospital, Mineóla, N. Y. F r a g m e n t a t i o n o f R e d C ells a n d G h o s ts in U re a S o lu tio n s , w ith a N o te o n E le c tr o p h o r e s is P a t t e r n s * By ERIC PONDER and RU TH PONDER Since the readiness with which red cells and ghosts break into fragments and give off myelin forms is an indication of the stability of their structure, any new fragmentation phenomenon is interesting. The one to be described here was met with during attempts to solu­ bilise the proteins (other than Hb) of red cell ghosts by the addition of urea. This denaturant keeps some denatured proteins in solution; it is believed that it liberates sulfhvdryl groups by bringing about an unfolding of the native protein molecule, which loses its rigid steric configuration and becomes more randomly arranged (Putnam, 1948). The procedure http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Haematologica Karger

Fragmentation of Red Cells and Ghosts in Urea Solutions, with a Note on Electrophoresis Patterns

Acta Haematologica , Volume 12 (4): 9 – Jan 1, 2009

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Publisher
Karger
Copyright
© 1954 S. Karger AG, Basel
ISSN
0001-5792
eISSN
1421-9662
DOI
10.1159/000204630
Publisher site
See Article on Publisher Site

Abstract

P onder, E. and R . P onder: Acta hacm at. 12, 282 -290, 1934. From T he Nassau Hospital, Mineóla, N. Y. F r a g m e n t a t i o n o f R e d C ells a n d G h o s ts in U re a S o lu tio n s , w ith a N o te o n E le c tr o p h o r e s is P a t t e r n s * By ERIC PONDER and RU TH PONDER Since the readiness with which red cells and ghosts break into fragments and give off myelin forms is an indication of the stability of their structure, any new fragmentation phenomenon is interesting. The one to be described here was met with during attempts to solu­ bilise the proteins (other than Hb) of red cell ghosts by the addition of urea. This denaturant keeps some denatured proteins in solution; it is believed that it liberates sulfhvdryl groups by bringing about an unfolding of the native protein molecule, which loses its rigid steric configuration and becomes more randomly arranged (Putnam, 1948). The procedure

Journal

Acta HaematologicaKarger

Published: Jan 1, 2009

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