Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Enhanced fluorescence‐based bio‐detection through selective integration of reflectors in microfluidic lab‐on‐a‐chip

Enhanced fluorescence‐based bio‐detection through selective integration of reflectors in... Purpose – This paper proposes to examine a simple and cost‐effective method of integrating a reflector surface with a silicon‐based microfluidic channel for enhanced biosensing through the method of fluorescence in a microfluidics and nanofluidics‐based lab‐on‐a‐chip device. Design/methodology/approach – Herein, the reflector is integrated with silicon‐based microfluidic channels and fluorescence measurements were carried out using alexafluor 647 particles. Two types of microfluidic channel surfaces were used, with and without reflector integration, for the experiments. Findings – The experimental results prove that the proposed technique of partial reflector integration within microfluidic or nanofluidic channel surfaces is highly suitable for fluorescence‐based detection of single molecules and low concentration fluorophore‐tagged receptors. Originality/value – It is believed that this is a novel work of integrating a reflector with a microfluidic channel surface for fluorescence‐based biodetection. This method will be very useful for fluorescence‐based biosensors in detecting low concentration fluorophores and single molecules. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Sensor Review Emerald Publishing

Enhanced fluorescence‐based bio‐detection through selective integration of reflectors in microfluidic lab‐on‐a‐chip

Arvind Chandrasekaran; Muthukumaran Packirisamy
Sensor Review , Volume 28 (1): 6 – Jan 25, 2008
Download PDF
6 pages

Loading next page...
 
/lp/emerald-publishing/enhanced-fluorescence-based-bio-detection-through-selective-JiCf1wx0kB

References (16)

Publisher
Emerald Publishing
Copyright
Copyright © 2008 Emerald Group Publishing Limited. All rights reserved.
ISSN
0260-2288
DOI
10.1108/02602280810850008
Publisher site
See Article on Publisher Site

Abstract

Purpose – This paper proposes to examine a simple and cost‐effective method of integrating a reflector surface with a silicon‐based microfluidic channel for enhanced biosensing through the method of fluorescence in a microfluidics and nanofluidics‐based lab‐on‐a‐chip device. Design/methodology/approach – Herein, the reflector is integrated with silicon‐based microfluidic channels and fluorescence measurements were carried out using alexafluor 647 particles. Two types of microfluidic channel surfaces were used, with and without reflector integration, for the experiments. Findings – The experimental results prove that the proposed technique of partial reflector integration within microfluidic or nanofluidic channel surfaces is highly suitable for fluorescence‐based detection of single molecules and low concentration fluorophore‐tagged receptors. Originality/value – It is believed that this is a novel work of integrating a reflector with a microfluidic channel surface for fluorescence‐based biodetection. This method will be very useful for fluorescence‐based biosensors in detecting low concentration fluorophores and single molecules.

Journal

Sensor Review – Emerald Publishing

Published: Jan 25, 2008

Keywords: Biotechnology; Fluidics; Reflection

There are no references for this article.