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Oxygen-regulated gene expression in murine cumulus cells

Oxygen-regulated gene expression in murine cumulus cells Oxygen is an important component of the environment of the cumulusoocyte complex (COC), both in vivo within the ovarian follicle and during in vitro oocyte maturation (IVM). Cumulus cells have a key role in supporting oocyte development, and cumulus cell function and gene expression are known to be altered when the environment of the COC is perturbed. Oxygen-regulated gene expression is mediated through the actions of the transcription factors, the hypoxia-inducible factors (HIFs). In the present study, the effect of oxygen on cumulus cell gene expression was examined following in vitro maturation of the murine COC at 2, 5 or 20 oxygen. Increased expression of HIF-responsive genes, including glucose transporter-1, lactate dehydrogenase A and BCL2/adenovirus E1B interacting protein 3, was observed in cumulus cells matured at 2 or 5, compared with 20 oxygen. Stabilisation of HIF1 protein in cumulus cells exposed to low oxygen was confirmed by western blot and HIF-mediated transcriptional activity was demonstrated using a transgenic mouse expressing green fluorescent protein under the control of a promoter containing hypoxia response elements. These results indicate that oxygen concentration influences cumulus cell gene expression and support a role for HIF1 in mediating the cumulus cell response to varying oxygen. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reproduction Fertility and Development CSIRO Publishing

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References (65)

Publisher
CSIRO Publishing
Copyright
Copyright © The Author(s). Published by CSIRO Publishing
ISSN
1031-3613
eISSN
1448-5990
DOI
10.1071/RD13249
pmid
24388334
Publisher site
See Article on Publisher Site

Abstract

Oxygen is an important component of the environment of the cumulusoocyte complex (COC), both in vivo within the ovarian follicle and during in vitro oocyte maturation (IVM). Cumulus cells have a key role in supporting oocyte development, and cumulus cell function and gene expression are known to be altered when the environment of the COC is perturbed. Oxygen-regulated gene expression is mediated through the actions of the transcription factors, the hypoxia-inducible factors (HIFs). In the present study, the effect of oxygen on cumulus cell gene expression was examined following in vitro maturation of the murine COC at 2, 5 or 20 oxygen. Increased expression of HIF-responsive genes, including glucose transporter-1, lactate dehydrogenase A and BCL2/adenovirus E1B interacting protein 3, was observed in cumulus cells matured at 2 or 5, compared with 20 oxygen. Stabilisation of HIF1 protein in cumulus cells exposed to low oxygen was confirmed by western blot and HIF-mediated transcriptional activity was demonstrated using a transgenic mouse expressing green fluorescent protein under the control of a promoter containing hypoxia response elements. These results indicate that oxygen concentration influences cumulus cell gene expression and support a role for HIF1 in mediating the cumulus cell response to varying oxygen.

Journal

Reproduction Fertility and DevelopmentCSIRO Publishing

Published: Jan 6, 2014

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